B. hydrolyticusの纎維素に對する擧動研究 : 細菌Hydrolaseに關する研究 : (第2報)

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Growth of B. hydorlyticus (Bh) on cellulose-media. such as modified OMELIANSKY'S medium, is accelerated by association with B. megalocellum n. sp., (Bm) which has been isolated from manure. Morphological and physiological characterisitcs of Bm have already been described. In cultures of Bh associated with Bm, changes in the appearance of filter paper in the media are regarded mainly due to the action of Bh, although Bm can grow singly on O's medium.Characteristics of associated cultures, especially as to the adaptation and modes of action on cellulose were investigated. In cultures on modified O's medium, diminution of cellulose is not remarkable, not exceeding 5% of added quantities even in 15 day old cultures. However, the residual cellulose is found to become fragile as compared with sterile controls. For instance, the sulfuric acid concentration necessary to exhibit color reaciton with l_2,markedly lowered with the "residual cellulose". For thenquantitative expression of the enzymic action of such mode, we adopted properly an index(R) defined as follows : R=100・(K-K_0)/K_0where K is a velocity constant of hydrolysis of residual cellulose with n-H_2SO_4 at 100℃, and K_0 is that of intact one in a control. R ranged 43〜93 in 15 day old cultures at 30℃, depending on the cmpositions of media.It may be suggested that such a mode of attack described here plays an important role in the "weathering" and decomposition of cellulose materials in nature, although attention has been focused by most researchers on another mode of action, i. e. liquefaction or decomposition to soluble low-molecular substances. It seems likely that there are at least two different types in so-called cellulase, one being the liquefying or "saccharogen" type, which has been studies extensively by PRINGSHEIM, KARRER and others, and another being, so to say, the "weathering" type as shown in this paper. Enzymological researches will be presented shortly.
公益社団法人日本生物工学会の論文
1949-03-15

B. hydrolyticusの纎維素に對する擧動研究 : 細菌Hydrolaseに關する研究 : (第2報)

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