酵母によるアントラニン酸よりトリプトファンの醗酵生産 (第2報) : 培養条件の検討とL-トリプトファンの確認

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An isolate, Fe-36,belonging to Candida and several strains, H095 etc., selected from among stock cultures of Hansenula yeasts were employed. These strains were capable of accumulating tryptophan in considerable amounts from anthranilic acid added as a precursor to a medium having adequate composition. As nitrogen sources, ammonium salt and urea were effective, and, as carbon sources, glucose, glycerol and sucrose were almost equally effective for accumulating tryptophan. Addition of calcium carbonate was effectual for maintaining medium pH within a range adequate for tryptophan production. The time-course of submerged cultures in a jar fermentor indicated that the tryptophan formation belongs to the type of differential synthesis : specific growth rate, respiratory activity (Qo_2) and the specific rate of tryptophan formation (τ) run almost parallel to each other. But, a possibility was suggested from some instances that the time-course could be converted partially into that of preferential synthesis.Concentration of dissolved oxygen suitable for maintaining τ-values at high levels for a long period was shown to be ca. 0.04 mM or a little lower. In adequate conditions, more than 90 per cent of anthranilic acid consumed was converted to tryptophan. The highest yiels hitherto obtained was 1.6g per liter of medium.Tryptophan was isolated from culture filtrate by adsorption on active carbon followed by elution with ethanol. Recrystallized preparation after being subjected to bioassay employing tryptophanless mutant of Saccharomyces yeast and to the measurements of physical constants, was proved to be of L-form.
公益社団法人日本生物工学会の論文
1962-05-15

酵母によるアントラニン酸よりトリプトファンの醗酵生産 (第2報) : 培養条件の検討とL-トリプトファンの確認

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