土壌より分離した酵母によるアントラニル酸の分解代謝について

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Dissimilative metabolism of anthranilate by a yeast strain isolated from soil was investiated.1. Several yeast strains capable of dissimilating anthranilate aerobically were isolated from soil. Morphorogical and physiological properties of E-1 strain having the highest activity of anthranilate oxidation led to the conclusion that it belongs to Trichosporon cutaneum.2. Anthranilate induced cells of E-1 had a pH-optimum for anthranilate oxidation at 5.0 (Qo_2=170). The optimal pH for anthranilate induction, however, was at 6.0.3. In the presence of α, α'-dipyridyl (5×10^-4M), 3-hydroxyanthranilate, 2,3-dihydroxybenzoate and catechol were accumulated aerobically in the culture medium added initially with anthranilate.4. The anthranilate-induced intact cells of E-1 strain oxidized 2,3-dihydroxybenzoic acid, but was incapable of oxidizing 3-hydroxyanthranilate or catechol. The extracts from the cells showed activities of 2,3-dihydroxybrobenzoic acid decarboxylase and catechol oxidase which had been induced.5. The extract of cells precviously induced by 3-hydroxyanthranilate also had the activities of the described decarboxylase and oxidase.The metabolic features of anthranilate in E-1 strain were compared with those in Aspergillus niger, presented in the previous report. The following scheme was postulated for the pathway of anthranilate dissimilation by the yeast strain : anthranilic acid →3-hydroxyanthranilic acid →2,3-dihydroxybenzoic acid →catechol →[ring cleavage]
公益社団法人日本生物工学会の論文
1961-12-15

土壌より分離した酵母によるアントラニル酸の分解代謝について

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