Aspergillus niger のBiochemical mutant によるアントラニル酸の代謝について

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The present investigation was undertaken in order to obtain information on the role of tryptophan pathway in the dissimilation by Aspergillus niger of anthranilate leading to the formation of 3-hydroxyanthranilate, 2,3-dihydroxybenzoate and catechol as intermediates. The results obtained are summarized as follows.1. Mutant A23,derived from the original strain by U.V. irradiation required for growth anthranilate which could be substituted by tryptophan, indole, nicotinate or 3-hydroxyanthranilate, indicating the incapability of anthranilate biosynthesis.2. Mutant K1,derived from A23,showed no growth when anthranilate (or nicotinate) was substituted by tryptophan, but the latter could stimulate growth when it was restricted due to the insufficient supply of nicotinate. Mycelia of K1 strain could take up tryptophan, though not remarkably, from the medium. These results indicated that tryptophan was permeable to the mycelia of K1 despite the lack of the pathway from tryptophan to nicotinate in this mutant.3. Mutant AG1 derived from A23 and mutant K1 dissimilated anthranilate adaptively. Anthranilate-induced mycelia of these strains contained 2,3-dihydroxybenzoic acid decroboxylase and catechol oxidase which were demonstrated in cell-free extracts.4. Tryptophan could induce the above two enzymes in AG1 mycelia, but not in K1 mycelia.5. The pathway of anthranilate dissimilation in K1 mutant was presumed therefore to involve the direct hydroxylation of the substrate to form 3-hydorxyanthranilate ; the possibility of tryptophan pathway was thus excluded in the case of this strain. Data presented also indicated that the roundabout pathway via tryptophan plays no significant role in the active dissimilation of anthranilate by the other strains of Asp. niger employed.
公益社団法人日本生物工学会の論文
1961-12-15

Aspergillus niger のBiochemical mutant によるアントラニル酸の代謝について

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