SP-2300 を液相としたガスクロマトグラフィーによる脂肪酸組成の分析

概要

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On the analysis of fatty acid compositions of vegetable oils such as rapeseed oil and peanut oil by GLC, the use of a packed column with SP-2300 thermostable liquid phase was effective as one of the solutions of the difficulty due to chain-length overlap between methyl linolenate and arachidate or eicosenoate. The retention time of methyl derivatives above C<SUB>20</SUB> on SP-2300 column was much faster, because of high temperature operating, than that on polar polyester or organosilicone polyester phases used commonly in fatty acid analysis. For example, methyl linolenate, arachidate, eicosenoate and tetracosenoate were eluted in 8.0, 8.8, 9.4 and 18.7min, respectively, by a temperature programming from 180 to 240°C at a rate of 4°C/min on a stainless steel column (3mm×2.25m) packed with 5% SP-2300 on 6080mesh Chromosorb W AWDMCS or on 6080mesh Celite 545 SKDMCS. The quantitative analysis of a standard mixture of fatty acid methyl esters for GLC on the above two columns showed that the percentage of the peak area for each fatty acid ester except methyl linolenate agreed closely with that of weight of the corresponding fatty acid ester, and that no correction of detector response was needful even in long chain esters containing 20 or more carbon atoms for usual analyses.
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