金属-タンパク質複合体に関する研究 (第6報) : 酵素タンパク質と金属との反応

概要

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Pancreatic lipase was purified by dialysis, acetone fractionation, methanol washing and trichloroacetic acid. The reaction of purified lipase and metals was conducted in water at 37°C for 20 hours and obtained its complex. The complex was purified by methanol washing. The metal content and IR spectrum of isolated complex were determined.<BR>From the experimental results, it was found that the formation of lipase-metal complex was easy as well as casein-metal complex. The complex showed the same pattern as the lipase or metal after gel filtration though Sephadex G75, but the metal in lipase-metal complex was not removed by dialysis, acetone fraction and methanol washing etc.. Therefore it seems clear that such a binding between lipase and metal is strong.
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