Lipid production in Porphyridium cruentum grown under different culture conditions(BIOCHEMICAL ENGINEERING)

概要

論文の詳細を見る
Autotrophic growth of Porphyridium cruentum under 18:12h and 12:12h light:dark cycles showed the maximum cell concentration of 2.1g-dry wt./L, whereas the specific growth rate, 0.042 (1/h), at 18:6h is faster than that of 12:12h, 0.031 (1/h), respectively. The highest lipid accumulation level, 19.3 (%, w/w), was achieved at 12:12h cycle. Under dark cultivation condition with 10g/L of glucose, the lipid accumulation in the cell was 10.9 (%, w/w), whereas the heterotrophic growth with glycerol as the carbon resource showed low level of cell concentration and lipid production, compared to that of glucose. The glucose was decided to be a suitable carbon resource for the heterotrophic growth of P. cruentum. The lipids from P. cruentum seemed be feasible for biodiesel production, because over 30% of the lipid was C_<16>-C_<18:1>. The cultivation time and temperature were important factors to increase the maximum cell concentration. Extending the cultivation time helps maintain the maximum cell concentration, and higher lipid accumulation was achieved at 25℃, compared to 35℃. The fed-batch cultures showed that, under the light condition, the specific production rate was slightly decreased to 0.4% lipid/g-dry wt./day at the later stage, whereas, under the dark condition, the specific production rate was maintained to be a maximum value of 1.1% lipid/g-dry wt./day, even in the later stage of cultivation. The results indicate that the heterotrophic or 12:12h cyclic mixotrophic growth of P. cruentum could be used for the production of biodiesel in long-term fed-batch cultivation of P. cruentum.

Lipid production in Porphyridium cruentum grown under different culture conditions(BIOCHEMICAL ENGINEERING)

スポンサーリンク

概要

著者

関連論文

スポンサーリンク