Long-term outdoor cultivation by perfusing spent medium for biodiesel production from Chlorella minutissima(BIOCHEMICAL ENGINEERING)
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概要
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A unique perfusion process was developed to maintain high concentrations of marine alga, Chlorella minutissima. This method is based on recycling cells by continuous feeding with warm spent sea water from nuclear power plants, which has very similar properties as sea water. A temperature of at least 30℃ in a 200L photo-bioreactor was maintained in this system by perfusion of the thermal plume for 80 days in the coldest season. The maximum cell concentration and total lipid content was 8.3g-dry wt./L and 23.2%, w/w, respectively, under mixotrophic conditions. Lipid production was found to be due to a partially or non-growth related process, which implies that large amounts of biomass are needed for a high accumulation of lipids within the cells. At perfusion rates greater than 1.5L/h, the temperature of the medium inside the reactor was around 30℃, which was optimal for cell growth. For this system, a perfusion rate of 2.8L/h was determined to be optimal for maintaining rapid cell growth and lipid production during outdoor cultivation. It was absolutely necessary to maintain the appropriate perfusion rate so that the medium temperature was optimal for cell growth. In addition, the lipids produced using this process were shown to be feasible for biodiesel production since the lipid composition of C. minutissima grown under these conditions consisted of 17% (w/w) of C_<16> and 47% (w/w) of C_<18>. The combined results of this study clearly demonstrated that the discharged energy of the thermal plume could be reused to cultivate marine alga by maintaining a relatively constant temperature in an outdoor photo-bioreactor without the need for supplying any extra energy, which could allow for cheap production of biodiesel from waste energy.
著者
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Oh Sung
College of Bioscience and Biotechnology, Department of Biomaterials Engineering, Kangwon National Un
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Kwon Min
College of Bioscience and Biotechnology, Department of Biomaterials Engineering, Kangwon National Un
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Choi Woon
College of Bioscience and Biotechnology, Department of Biomaterials Engineering, Kangwon National Un
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Seo Yong
College of Bioscience and Biotechnology, Department of Biomaterials Engineering, Kangwon National Un
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Kim Ga
College of Engineering, Department of Biological Engineering, Kangwon National University
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Kang Do
Korea Ocean Research & Development Institute
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Lee Shin
College of Engineering, Department of Biological Engineering, Kangwon National University
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Lee Hyeon
College of Bioscience and Biotechnology, Department of Biomaterials Engineering, Kangwon National Un
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Oh Sung
College Of Bioscience And Biotechnology Department Of Biomaterials Engineering Kangwon National Univ
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Lee Shin
College Of Engineering Department Of Biological Engineering Kangwon National University
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Seo Yong
College Of Bioscience And Biotechnology Department Of Biomaterials Engineering Kangwon National Univ
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Lee Hak
Division Of Wood Chemistry & Microbiology Korea Forest Research Institute
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Lee Hyeon
College Of Bioscience And Biotechnology Department Of Biomaterials Engineering Kangwon National Univ
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Kwon Min
College Of Bioscience And Biotechnology Department Of Biomaterials Engineering Kangwon National Univ
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Kang Do
Korea Ocean Research & Development Institute
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Lee H
College Of Bioscience And Biotechnology Department Of Biomaterials Engineering Kangwon National Univ
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Choi Woon
College Of Bioscience And Biotechnology Department Of Biomaterials Engineering Kangwon National Univ
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Kim Ga
College Of Engineering Department Of Biological Engineering Kangwon National University
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