Effect of different extraction protocols on anticancer and antioxidant activities of Berberis koreana bark extracts(METHODS)

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概要

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• High-pressure extraction and ultrasonification extraction techniques were employed to extract bioactive compounds from Berberis koreana. This study aimed to determine the effect of ultrasonification in a high pressure process on the extraction yield, and the anticancer and antioxidant activities of the B. koreana bark extract. The effect of high-pressure extraction time when carried out for 5 and 15min(HP5 and HP15) was also investigated. The best extraction yield with maximum percentage of phenolic compounds was obtained using high pressure with sonification(HPWS) extraction method. Experimental results indicated that HPWS altered the antioxidant activities, including the scavenging capacity of diphenylpicrylhydrazyl(DPPH) and xanthine oxidase. HP5 and HP15 with conventional extraction have almost similar bioactivity, but showed lower antioxidant and anticancer activities compared to HPWS. The results showed that the application of ultrasonification improved the extraction efficiency for bioactive compounds and, as deduced from chromatographic profiles, it may have allowed the release of new compounds. The scanning electron microscope(SEM) showed evidence of rupturing of the tissue surface treated with HPWS, in contrast to conventional extraction, HP5, and HP15. The HPWS extraction was not only more efficient but also convenient for the recovery and purification of the active compounds of hard plant tissues.

• 社団法人日本生物工学会の論文

著者

• Han Jae

  Division Of Bio Engineering College Of Bioscience And Biotechnology Kangwon National University

• Ha Ji

  Division Of Bio Engineering College Of Bioscience And Biotechnology Kangwon National University

• Qadir Syed

  Division of Bio Engineering, College of Bioscience and Biotechnology, Kangwon National University

• Kwon Min

  Division of Bio Engineering, College of Bioscience and Biotechnology, Kangwon National University

• Chung Hyang

  Division of Bio Engineering, College of Bioscience and Biotechnology, Kangwon National University

• Ahn Juhee

  Division of Bio Engineering, College of Bioscience and Biotechnology, Kangwon National University

• Lee Hyeon

  Division of Bio Engineering, College of Bioscience and Biotechnology, Kangwon National University

• Lee Hyeon

  College Of Bioscience And Biotechnology Department Of Biomaterials Engineering Kangwon National Univ

• Ahn Juhee

  Division Of Bio Engineering College Of Bioscience And Biotechnology Kangwon National University

• Qadir Syed

  Division Of Bio Engineering College Of Bioscience And Biotechnology Kangwon National University

• Chung Hyang

  Division Of Bio Engineering College Of Bioscience And Biotechnology Kangwon National University

• Kwon Min

  Coll. Of Bioscience And Biotechnology Dep. Of Biomaterials Engineering Kangwon National Univ. Chunch

• Lee Hyeon

  Division Of Bio Engineering College Of Bioscience And Biotechnology Kangwon National University

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