Effect of Mycelial Culture Broth of Ganoderma lucidum on the Growth Characteristics of Human Cell Lines

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概要

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• Two types of purified samples, water-soluble(sample A ; M. W, 1.2×10^6 dalton)and water-in-soluble(sample C ; M. W., 1.0×10^6 dalton)samples, were obtained through consecutive separation processes from the culture broth of Ganoderma lucidia mycelium. It was found that both samples from the culture broth were very effective in inhibiting the growth of several human cancer cell lines, having a 93-85% growth inhibition oh Hep3B, AGS and A549 with the least cytotoxicity on the normal human lung cell line, WRL68 of less than 25% the highest supplementation concentration of 1.0mg/l. In general, the sample C showed greater inhibition of cancer cell growth than the sample A. The same trend was also observed in antimutagenicity using the Chinese hamster ovary cell line(CHO test)or Salmonella typhimurium(Ames test). The CHO test showed that sample C had higher antimutagenicity on mutagens 4NQO or MMNG than sample A(approximately 40% vs approximately 25%). The percentage of antimutagenicity from the Ames test was lower than that from the CHO test, possibly due to the difference in the sensitivity of mutagens. The water-insoluble sample greatly enhanced the growth of the human T cell line(H9)up to 1×10^5 with sample supplementation at 1.0mg/l concentration from 4.3×10^4 without sample supplementation as well as improved the secretion level of both IL-6 and TNF-α up to 100pg/ml from approximately 40pg/ml without sample supplementation. The kinetics of response to the immune cell growth was illustrated by the response time obrained when the sample concentration was increased. The water-insoluble sample can be used for effectively treating cancer in that it accelerated apoptosis of human carcinoma cells up to 70% compared to less than 50% for the control. The sample also increased the differentiation ratio of HL-60 cells up to 58% after four days of cultivation, compared to 18% in the case of no sample supplementation. These results can be used in implying that the insoluble part of G. lucidium mycelium culture broth must be related to controlling signal transduction, resulting in the regulation of cancer cell growth.

• 社団法人日本生物工学会の論文
• 2001-12-25

著者

• Lee Shin

  College of Engineering, Department of Biological Engineering, Kangwon National University

• Kim Jong

  Department Of Surgery College Of Medicine Hallym University

• Lee Shin

  College Of Engineering Department Of Biological Engineering Kangwon National University

• Kim Jong

  Division Of Mechanical Engineering Pukyong National University

• Lee Hyeon

  Division of Bio Engineering, College of Bioscience and Biotechnology, Kangwon National University

• CHUNG Woo

  Division of Applied Life Science (BK21), Environmental Biotechnology National Core Research Center a

• Lee Hak

  Division Of Wood Chemistry & Microbiology Korea Forest Research Institute

• Lee S

  Division Of Environmental And Biochemical Engineering Kangwon National University

• LEE SEO

  Division of Food and Biotechnology, Kangwon National University

• PARK YOUNG

  O. Z. Tech, Chuchon Bioventure Center

• HWANG BAIK

  Department of Biology, Chonnam National University

• LEE SHIN

  Division of Environmental and Biochemical Engineering, Kangwon National University

• Lee Seo

  Division Of Food And Biotechnology Kangwon National University

• Hwang Baik

  Department Of Biology Chonnam National University

• Chung Woo

  Division Of Food And Biotechnology Kangwon National University

• Lee H

  College Of Bioscience And Biotechnology Department Of Biomaterials Engineering Kangwon National Univ

• Kim Jong

  Division Of Biotechnology College Of Life Sciences And Biotechnology Korea University

• Lee Shin

  Division Of Animal Science And Technology Gyeongsang National University

• Lee Hyeon

  Division Of Bio Engineering College Of Bioscience And Biotechnology Kangwon National University

• Chung Woo

  Division Of Applied Life Science (bk21) Environmental Biotechnology National Core Research Center An

• Kim Jong

  Division Of Applied Life Science (bk21 Program) Gyeongsang National University

• Park Young

  O. Z. Tech Chuchon Bioventure Center

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