ent-Kaurane and ent-Pimarane Diterpenes from Siegesbeckia pubescens Inhibit Lipopolysaccharide-Induced Nitric Oxide Production in BV2 Microglia
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概要
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The extract of Siegesbeckia pubescens herb and its chemical constituents were tested for the ability to inhibit lipopolysaccharide (LPS)-induced nitric oxide (NO) production in BV2 microglia. The methanol extract and the 90% MeOH fraction of S. pubescens effectively attenuated lipopolysaccharide-induced nitric oxide production. Several steps of chromatography yielded eight ent-kaurane diterpenes (1–8) and one ent-pimarane diterpene (9) from the 90% MeOH fraction. Among these compounds, compounds 2–9 showed significant inhibitory effect on lipopolysaccharide-induced nitric oxide production in BV2 microglia. Compounds 3 and 9 concentration-dependently decreased the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), supported by quantitative real time polymerase chain reaction (PCR) and Western blot analysis. These results suggest that ent-kaurane and ent-pimarane diterpenes isolated from S. pubescens are expected to be potential candidates against neuroinflammation-related disease.
著者
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Kim Seung
College of Bioscience and Biotechnology, Department of Biomaterials Engineering, Kangwon National Un
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Lee Hee
Institute Of Animal Medicine College Of Veterinary Medicine Gyeongsang National University
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Sung Sang
College Of Pharmacy And Research Institute Of Pharmaceutical Science Seoul National University
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SUNG Sang
College of Pharmacy and Research Institute of Pharmaceutical Science, Seoul National University
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LEE Mina
College of Pharmacy and Research Institute of Pharmaceutical Science, Seoul National University
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Kim Seung
College of Pharmacy, Yonsei Institute of Pharmaceutical Sciences, Yonsei University
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Cho Yekyung
College of Pharmacy and Research Institute of Pharmaceutical Science, Seoul National University
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Kang Jimmy
College of Pharmacy and Research Institute of Pharmaceutical Science, Seoul National University
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Lee Hee
Institute for Life Science, Elcomscience Co., Ltd.
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- ent-Kaurane and ent-Pimarane Diterpenes from Siegesbeckia pubescens Inhibit Lipopolysaccharide-Induced Nitric Oxide Production in BV2 Microglia