Berberine Exerts Neuroprotective Actions against in Vitro Ischemia-Induced Neuronal Cell Damage in Organotypic Hippocampal Slice Cultures : Involvement of B-Cell Lymphoma 2 Phosphorylation Suppression(Pharmacology)
スポンサーリンク
概要
- 論文の詳細を見る
In this study we elucidated the effects of berberine, a major alkaloid component contained in medicinal herbs, such as Phellodendri Cortex and Coptidis Rhizoma, on ischemic neuronal damage in mouse organotypic hippocampal slice cultures (OHSCs) caused by oxygen and glucose deprivation (OGD) and N-methyl-D-aspartate (NMDA) -type glutamate receptor stimulation. Hippocampal slices obtained from 7-d-old ICR mice were cultured for 10d before the experiments. Ischemia-related damage was induced by OGD (5, 15, 45min) or NMDA (10μM) treatment, and was evaluated by measuring propidium iodide (PI) uptake. Levels of apoptotic marker proteins, B-cell lymphoma 2 (Bcl-2) and phosphorylated-Bcl-2 (p-Bcl-2), in the OHSCs were measured as indices of biochemical neuronal cell damage by Western blotting. Berberine (5, 25μM) or the NMDA antagonist MK-801 (25μM) was added to the medium 30min before OGD or NMDA treatment. OGD time-dependently increased PI uptake of the OHSCs. Both berberine (5, 25μM) and MK-801 (25μM) significantly inhibited PI uptake at 24h after 45-min OGD treatment and PI uptake in OHSCs exposed to NMDA for 24h. OGD treatment also significantly increased the level of p-Bcl-2 but not that of Bcl-2 or glyceraldehyde-3-phosphate dehydrogenase (GAPDH) in OHSCs. Berberine (5-25μM) significantly suppressed the OGD-induced increase of p-Bcl-2 level in OHSCs when tissue was exposed to the alkaloid prior to OGD or simultaneously with OGD. These findings suggest that berberine has protective effects against ischemic damage in mouse OHSCs and that the effects are at least partly mediated by suppression of Bcl-2 phosphorylation.
- 社団法人日本薬学会の論文
- 2009-01-01
著者
-
MURAKAMI Yukihisa
Division of Medicinal Pharmacology, Institute of Natural Medicine, Toyama Medical and Pharmaceutical
-
MATSUMOTO Kinzo
Division of Medicinal Pharmacology, Institute of Natural Medicine, Toyama Medical and Pharmaceutical
-
Murakami Yukihisa
富山大学和漢医薬学総合研究所 複合薬物薬理学
-
Mukarami Yukihisa
Institute Of Natural Medicine Toyama Medical And Pharmaceutical University
-
Murakami Yukihisa
Division Of Medicinal Pharmacology Institute Of Natural Medicine Toyama Medical And Pharmaceutical U
-
Murakami Yukihisa
Research Institute For Wakan-yaku (traditional Sino-japanese Medicines)
-
Murakami Yuko
Department Of Bioactive Molecules National Institute Of Infectious Diseases
-
Matsumoto Kinzo
Div. Of Medicinal Pharmacology Inst. Of Natural Medicine Univ. Of Toyama
-
Matsumoto Kinzo
Division Of Medicinal Pharmacology Institute Of Natural Medicine Toyama Medical And Pharmaceutical U
-
ZHAO Qi
Division of Medicinal Pharmacology, Institute of Natural Medicine,University of Toyama
-
HORI Hitomi
Division of Medicinal Pharmacology, Institute of Natural Medicine, University of Toyama
-
CUI Hu-Shan
Division of Medicinal Pharmacology, Institute of Natural Medicine, University of Toyama
-
OBI Ryosuke
Division of Medicinal Pharmacology, Institute of Natural Medicine, University of Toyama
-
Matsumoto K
Div. Of Medicinal Pharmacology Inst. Of Natural Medicine Univ. Of Toyama
-
Zhao Qi
Division Of Medicinal Pharmacology Institute Of Natural Medicine University Of Toyama
-
Zhao Qi
Division Of Pharmacology Institute Of Natural Medicine Toyama Medical & Pharmaceutical Universit
-
Matsumoto Kinzo
富山大学和漢医薬学総合研究所 複合薬物薬理学
-
Murakami Y
Department Of Bioactive Molecules National Institute Of Infectious Diseases
-
Cui Hu-shan
Division Of Medicinal Pharmacology Institute Of Natural Medicine University Of Toyama:ophthalmology
-
Obi Ryosuke
Division Of Medicinal Pharmacology Institute Of Natural Medicine University Of Toyama:department Of
-
Hori Hitomi
Division Of Medicinal Pharmacology Institute Of Natural Medicine University Of Toyama
-
Murakami Y
Toho Univ. Chiba Jpn
-
Obi Ryosuke
Division Of Medicinal Pharmacology Institute Of Natural Medicine University Of Toyama
-
Cui Hu-shan
Division Of Medicinal Pharmacology Institute Of Natural Medicine University Of Toyama
関連論文
- Enhanced expression of BCL2/adenovirus EIB 19-kDa-interacting protein 3 mRNA, a candidate for intrinsic depression-related factor, and effects of imipramine in the frontal cortex of stressed mice
- Effects of Manganese Complexes of Curcumin and Diacetylcurcumin on Kainic Acid-Induced Neurotoxic Responses in the Rat Hippocampus(Pharmacology)
- Hypouricemic Effects of Acacetin and 4,5-O-Dicaffeoylquinic Acid Methyl Ester on Serum Uric Acid Levels in Potassium Oxonate-Pretreated Rats(Pharmacology)
- Antiproliferative Activity of the Netherlands Propolis and Active Constituents
- Fischer Indolization and Its Related Compounds. XIX. Syntheses of Ethyl 4-Methoxy- and Ethyl 5-Methoxy-1-phenyl-3H-benz [e] indole-2-carboxylates
- Corymine Potentiates NMDA-Induced Currents in Xenopus Oocytes Expressing NR1a/NR2B Glutamate Receptors
- Enhanced Expression of BCL2/Adenovirus EIB 19-kDa-Interacting Protein 3 mRNA, a Candidate for Intrinsic Depression-Related Factor, and Effects of Imipramine in the Frontal Cortex of Stressed Mice
- The mRNA Expression Differences of RNA Editing Enzymes in Differentiated and Undifferentiated NG108-15 Cells
- Effects of Choto-san and Its Related Constituents on Endogenous Antioxidant Systems(Pharmacology)
- Antioxidant and Free Radical-Scavenging Activity of Choto-san and Its Related Constituents(Pharmacology)
- Fischer Indolization of Variously ortho-Substituted Phenylhydrazones (Fischer Indolization and Its Related Compounds. XXV)
- A NEW SYNTHESIS OF EUPOLAURAMINE FROM A BENZ [f] INDOLE DERIVATIVE
- Buds of Baccharis dracunculifolia : potent source of biologically active caffeoylquinic acids and labdane-type diterpenes of Brazilian propolis
- Inhibitory Effects of Corymine-Related Compounds on Glycine Receptors Expressed in Xenopus Oocytes
- Effects of Mitragynine on cAMP Formation Mediated by δ-Opiate Receptors in NG108-15 Cells
- Behavioral Studies on Alkaloids Extracted from the Leaves of Hunteria zeylanica
- Anticonvulsant Activity of Paeonimetabolin-I Adducts Obtained by Incubation of Paeoniflorin and Thiol Compounds with Lactobacillus brevis
- Effects of Paeoniflorin Derivatives on Scopolamine-Induced Amnesia Using a Passive Avoidance Task in Mice; Structure-Activity Relationship
- Synthetic Studies of Indoles and Related Compounds. XXVII. A New Synthesis of Crenatine from Ethyl Indole-2-carboxylate
- Fischer Indolization and Its Related Compounds. XVIII. Formation of Four Unexpected Angular Benz [e] indoles on Fischer Indolization of Ethyl Phenylpyruvate 2-[(1,4-Dimethoxy-2-naphthyl) hydrazone]
- Synthetic Studies on Indoles and Related Compounds. XXVI. The Debenzylation of Protected Indole Nitrogen with Aluminum Chloride. (2)
- Permanent Occlusion of Bilateral Internal Carotid Arteries Produces Cognitive Deficits in Two Learning Behavior Tasks
- Tetramethylpyrazine Improves Spatial Cognitive Impairment Induced by Permanent Occlusion of Bilateral Common Carotid Arteries or Scopolamine in Rats
- Anti-hypertensive and anti-stroke effects of Chrysanthemum extracts in stroke-prone spontaneously hypertensive rats
- Possible Involvement of Signal Transducers and Activators of Transcription 3 System on Depression in the Model Mice Brain
- Chotosan Enhances Macrophage Colony-Stimulating Factor mRNA Expression in the Ischemic Rat Brain and C6Bu-1 Glioma Cells(Biochemistry)
- Chotosan, a Kampo Formula, Ameliorates Chronic Cerebral Hypoperfusion-Induced Deficits in Object Recognition Behaviors and Central Cholinergic Systems in Mice
- Preventive Effect of Chotosan, a Kampo Medicine, on Transient Ischemia-Induced Learning Deficit Is Mediated by Stimulation of Muscarinic M_1 But Not Nicotinic Receptor(Pharmacology)
- Social Isolation Stress-Induced Oxidative Damage in Mouse Brain and Its Modulation by Majonoside-R2, a Vietnamese Ginseng Saponin(Pharmacology)
- The Effects of Choto-san on the mRNA Expression of Alzheimer's Disease Related Factors in the Permanent Ischemic Rat Brain(Pharmacology)
- Expression Changes of the mRNA of Alzheimer's Disease Related Factors in the Permanent Ischemic Rat Brain(Pharmacology)
- Evaluation of the Nitric Oxide Radical Scavenging Activity of Manganese Complexes of Curcumin and Its Derivative(Pharmacology)
- Serotonin 2C Receptor (5-HT2CR) mRNA Editing–Induced Down-Regulation of 5-HT2CR Function in Xenopus Oocytes: the Significance of Site C Editing
- Berberine Exerts Neuroprotective Actions against in Vitro Ischemia-Induced Neuronal Cell Damage in Organotypic Hippocampal Slice Cultures : Involvement of B-Cell Lymphoma 2 Phosphorylation Suppression(Pharmacology)
- Cytoprotective and Cytotoxic Effects of Curcumin : Dual Action on H_2O_2-Induced Oxidative Cell Damage in NG108-15 Cells
- Protective effect of Choto-san and its related constituents on hydrogen peroxide-induced cell damage in NG108-15 cells
- Ameliorative Effects of Paeoniflorin, a Major Constituent of Peony Root, on Adenosine A_1 Receptor-Mediated Impairment of Passive Avoidance Performance and Long-Term Potentiation in the Hippocampus
- Paeoniflorin, a Major Constituent of Peony Root, Reverses Muscarinic M_1-Receptor Antagonist-Induced Suppression of Long-Term Potentiation in the Rat Hippocampal Slice
- Diazepam binding inhibitor (DBI) gene expression in the brains of socially isolated and group-housed mice
- GTS-21, a Nicotinic Agonist, Attenuates Multiple Infarctions and Cognitive Deficit Caused by Permanent Occlusion of Bilateral Common Carotid Arteries in Rats
- Chronic Cerebral Hypoperfusion-Induced Neuropathological Changes in Rats
- Antihypertensive Potential and Mechanism of Action of Astaxanthin : III. Antioxidant and Histopathological Effects in Spontaneously Hypertensive Rats(Pharmacology)
- Antihypertensive Potential and Mechanism of Action of Astaxanthin : II. Vascular Reactivity and Hemorheology in Spontaneously Hypertensive Rats(Pharmacology)
- Developmental Changes in Serotonin 2C Receptor mRNA Editing in the Rat Cerebral Cortex and Primary Cultured Cortical Neurons(Pharmacology)
- Tacrine Improves Working Memory Deficit Caused by Permanent Occlusion of Bilateral Common Carotid Arteries in Rats
- Effect of Pilocarpine on Striatal Acetylcholine Release in Dopamine-Depleted Rats
- Effect of Japanese Angelica Root Extract on Pentobarbital-Induced Sleep in Group-Housed and Socially Isolated Mice:Evidence for the Central Action
- In Vitro Antioxidant Activity of Vietnamese Ginseng Saponin and Its Components
- The Possible Involvement of GABA_A Systems in the Antinarcotic Effect of Majonoside-R2, a Major Constituent of Vietnamese Ginseng, in Mice
- A Combination of Soft-Shell Turtle Powder and Essential Oil of a Unicellular Chorophyte Prevents Bone Loss and Decreased Bone Strength in Ovariectomized Rats(Pharmacognosy)
- A Wild-type Japanese Measles Virus Strain Inducing Predominant Early Down-regulation of CD46
- Molecular Cloning of a Complementary DNA for a Membrane Cofactor Protein (MCP, CD46)/Measles Virus Receptor on Vero Cells and Its Functional Characterization
- Four Di-O-caffeoyl Quinic Acid Derivatives from Propolis. Potent Hepatoprotective Activity in Experimental Liver Injury Models
- POTENT ANTIHEPATOTOXIC ACTIVITY OF DICAFFEOYL QUINIC ACIDS FROM PROPOLIS
- Fischer Indolization and Its Related Compounds. XXIV. Fischer Indolization of Ethyl Pyruvate 2-(2-Methoxyphenyl)phenylhydrazone
- Fischer Indolization and Its Related Compounds. XXIII. Fischer Indolization of Ethyl Pyruvate 2-(2,6-Dimethoxyphenyl)phenylhydrazone
- Synthesis of Decalin Type Chiral Synthons Base on Enzymatic Function
- Caffeic Acid Phenethyl Ester (CAPE) Analogues: Potent Nitric Oxide Inhibitors from the Netherlands Propolis
- Biological Evaluation and LC-MS Analysis of Propolis from Brazil, Peru, China and the Netherlands : 天然物化学
- Constituents of Water Extract of Brazilian Propolis, Their Biological Properties and Botanical Origin
- Isolation of Two New Flavonoids and Antiproliferative Activity of Chinese Propolis
- Facilitatory and Inhibitory Effects of Harmaline on the Tryptophan- Induced 5-Hydroxytryptamine Syndrome and Body Temperature Changes in Pargyline-Pretreated Rats
- γ-Herpesviruses and cellular signaling in AIDS-associated malignancies
- Effect of Herbimycin A on Tyrosine Kinase Receptors and Platelet Derived Growth Factor (PDGF)-Induced Signal Transduction
- Antihypertensive and Neuroprotective Effects of Astaxanthin in Experimental Animals(Pharmacology)
- Influence of an Adenosine Deaminase Inhibitor, Erythro-9-(2-hydroxy-3-nonyl) Adenine Hydrochloride, on 5-HT2CR mRNA Editing in Primary Cultured Cortical Cells
- Paeoniflorin ameliorates acquisition impairment of a simple operant discrimination performance caused by unilateral nucleus basalis magnocellularis lesion in rats
- A Study for Palladium-catalyzed Chemoselective Vinylation at C-3 Position of 4-Bromo-1-tosylindole
- Substituent Effects in the Fischer Indolization of (2-Sulfonyloxyphenyl)-hydrazones (Fischer Indolization and Related Compounds . XXX)
- Neuropharmacological Actions of Pluchea indica LESS Root Extract in Socially Isolated Mice
- β-Acylation of Ethyl Pyrrole-2-carboxylate by Friedel-Crafts Acylation : Scope and Limitations (Synthetic Studies on Indoles and Related Compounds. XXXVIII)
- New Strategy for Indole Synthesis from Ethyl Pyrrole-2-carboxylate (Synthetic Studies on Indoles and Related Compounds. XXXIX)
- Serotonin 2C Receptor (5-HT2CR) mRNA Editing-Induced Down-Regulation of 5-HT2CR Function in Xenopus Oocytes : the Significance of Site C Editing
- 2-Chloro-N, N-dibenzoylaniline : a Selective N-Benzoylating Reagent
- Berberine Exerts Neuroprotective Actions against in Vitro Ischemia-Induced Neuronal Cell Damage in Organotypic Hippocampal Slice Cultures : Involvement of B-Cell Lymphoma 2 Phosphorylation Suppression
- Kampo Formulations, Chotosan, and Yokukansan, for Dementia Therapy: Existing Clinical and Preclinical Evidence