Effect of Pilocarpine on Striatal Acetylcholine Release in Dopamine-Depleted Rats

元データ 1997-01-15 公益社団法人日本薬学会

概要

We have previously demonstrated that the systemic administration of pilocarpine stimulates striatal acetylcholine (ACh) release in rats using a brain microdialysis technique. In the present study, we investigated whether a nigro-striatal dopaminergic system is involved in the pilocarpine-induced increase in striatal ACh release using dopamine-depleted rats under urethane anesthesia. The application of pilocarpine (0.1-10 mM) via the microdialysis tube increased striatal ACh release in normal rats in a concentration-dependent manner, but it had no effect on the release of glutamate or γ-aminobutyric acid (GABA) from the striatum. The increase in striatal ACh release caused by pilocarpine (1 mM) was enhanced by reserpine and α-methyl-p-tyrosine treatment, which completely depleted dopamine in the striatum. These results suggest that pilocarpine selectively increases striatal ACh release by acting at the striatum, and that the nigro-striatal dopaminergic neurons play an inhibitory role in the pilocarpine-induced ACh release.

著者

渡辺 裕司 International Research Center For Traditional Medicine Toyama Prefecture International Health Comple
Watanabe Hiroshi Department of Cardiology, Niigata City General Hospital
MURAKAMI Yukihisa Department of Pharmacology, Institute of Natural Medicine, Toyama Medical and Pharmaceutical Univers
MATSUMOTO Kinzo Department of Pharmacology, Institute of Natural Medicine, Toyama Medical and Pharmaceutical Univers
Murakami Yukihisa 富山大学和漢医薬学総合研究所 複合薬物薬理学
Mukarami Yukihisa Institute Of Natural Medicine Toyama Medical And Pharmaceutical University
Murakami Yukihisa Division Of Medicinal Pharmacology Institute Of Natural Medicine Toyama Medical And Pharmaceutical U
Murakami Yukihisa Research Institute For Wakan-yaku (traditional Sino-japanese Medicines)
Murakami Yukihisa Department Of Pharmacology Institute Of Natural Medicine:21st Century Coe Program Toyama Medical And
Matsumoto Kinzo Div. Of Medicinal Pharmacology Inst. Of Natural Medicine Univ. Of Toyama
Matsumoto Kinzo Department Of Pharmacology Institute Of Natural Medicine:21st Century Coe Program Toyama Medical And
Matsumoto Kinzo 富山大学和漢医薬学総合研究所 複合薬物薬理学
Murakami Y Department Of Bioactive Molecules National Institute Of Infectious Diseases
Watanabe Hiroshi Department Of Applied Chemistry And Bioengineering Graduate School Of Engineering Osaka City Univers

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