Oerskovia sp.の生産するβ-1,3-Glucanaseの精製とその諸性質について

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The culture filtrate of the CK strain, belonging to the genus Oerskovia, exhibited high lytic activity toward logarithmic or stationary phase cells of many species of yeast, when the CK strain was cultivated in the medium containing yeast cells. Chromatographic fractionation of the lytic culture fluid showed the presence of three types of β-1,3-glucanases, designated as F-0,F-2,and F-L. Each of them was purified through chromatographies on ECTEOLA- and CM-cellulose and gel filtration. Each preparation of enzymes was homogeneous by disc polyacrylamide gel electrophoresis at pH 9.4 and pH 4.0.F-0 had molecular weight near 29,000 and showed strong β-1,3-glucanase activity, but very weak lytic activity toward living yeast cells. Its optimum pH and temperature were 6.5 and 50°-55℃. The activity was lost on treatment at 60℃ for 15 min. The enzyme liberated predominantly laminaripentaose from yeast glucan.F-2 had molecular weight near 20,000 and showed strong β-1,3-glucanase activity, but the lytic activity was not detected in the enzyme fraction. Its optimum pH and temperature were 5.5 and 50°-55℃. The activity was lost on treatment at 60℃ for 15 min. The enzyme liberated glucose and laminaribiose.F-L had molecular weight near 12,000 and showed little β-1,3-glucanase activity but extremely high lytic activity. Its optimum pH and temperature of β-1,3-glucanase activity were 6.0 and 45°-50℃, and of lytic acitivity 7.5 and 35℃ respectively. The activity was lost on treatment at 60℃ for 15 min. The enzyme liberated oligosacharides, judged to be laminaritetraose and laminaripentaose, from yeast glucan. Each enzyme was stable at pH 6.0-9.0 and severely inhibited by 10^<-4> M HgCl_2.
社団法人日本生物工学会の論文
1976-09-25

Oerskovia sp.の生産するβ-1,3-Glucanaseの精製とその諸性質について

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