Saccharomyces cerevisiaeの高級アルコール生成と窒素源の影響

概要

論文の詳細を見る
Qualitative and quantitative effects of nitrogen source on higher alcohol formation were studied mostly with a leucine-requiring mutant of Saccharomyces cerevisiae which could form isoamylalcohol only from leucine. Ammonium sulfate and some amino acids, namely isoleucine, valine, phenylalanine and methionine, coordinately repressed isoamylalcohol formation from leucine via the Ehrlich pathway, although neither was inhibitory by itself unless the concentration was very high. Amino acids other than lysine, histidine, arginine, proline and glycine were also slightly inhibitory in the presence of ammonium sulfate. On the other hand, leucine coordinated with ammonium sulfate to repress isobutanol formation from valine and active amylalcohol formation from isoleucine. When sodium glutamate was added instead of ammonium sulfate, coordinate inhibition was a much lesser extent than ammonium sulfate, whereas glutamate itself was much more inhibitory than ammonium sulfate at higher concentrations. Competition between leucine, valine and isoleucine for the higher alcohol formation was generally found in all strains tested of both mutant and wild type when the ammonium-supplemented medium was fermented. The mode of isoamylalcohol formation in the medium with increasing amounts of casamino acid indicated that higher alcohol formation in medium containing a complex nitrogen soruce was regulated mainly through the Ehrlich pathyway. From these findings and the results of previous study, we conclude that higher alcohol formation depends in complex manner on both the quality and quantity of nitrogen source, and that the Ehrlich pathwas is important in the regulation of higher alcohol formation in medium with a complex nitrogen source.
1981-01-25

Saccharomyces cerevisiaeの高級アルコール生成と窒素源の影響

スポンサーリンク

概要

著者

関連論文

スポンサーリンク