協会7号酵母およびその泡なし変異株からProteinase処理によって遊離する細胞表層物質の分析

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A non-foaming mutant of sake yeast (Saccharomyces cerevisiae) quite differs from its parents strain, Kyokai No. 7,in its physicochemical properties, whereas no obvious difference could be found in their cell wall composition when whole cell wall preparations were analyzed. In this work, analyses were made on the cell surface materials released by trypsin, by which treatment the affinity of the cells for air bubbles was decreased, to detect differences between the parent and mutant cells in their yields, general compositions, amino acid compositions, chromatograms on Sephadex and DEAE-cellulose columns, and electrophoretic patterns on polyacrylamide gel. No particular difference was detected in all cases. It was also found that the trypsin-released meterials contained some additional hydrophobic amino acids compared with the whole cell wall. Chymotrypsinreleased materials were also prepared from cells which were pretreated with hot water to reduce the non-specifically released materials, and fractionated on Sephadex G-25 and G-50. These fractions were also richer in some hydrophobic amino acids than those of the whole cells in both the parent and mutant strains. These results support the conclustion that a modified steric structure of the mutant cell wall is attributable to the substitution of one or two amino acids in the constitutional cell wall protein, and were consistent with the idea that there exist some hydrophobic proteins in the cell surface of both parent and mutant cells.
社団法人日本生物工学会の論文
1975-03-25

協会7号酵母およびその泡なし変異株からProteinase処理によって遊離する細胞表層物質の分析

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