醸造酵母からの栄養要求性変異株の分離

概要

論文の詳細を見る
Auxotrophic mutants from a sake yeast having diploid or higher ploidy were isolated by the nystain enrichment method. To facilitate the isolation, canavanine resistant auxotrophic cells were mixed with the sake yeast before addition of nystatin to estimate the enrichment ratio in the nystatin treatment. Auxotrophic mutants of strain Kyokai no. 7 were found to need much higher enrichment for isolation than strain 7H3 (a haploid of Kyokai no. 7) as estimated from the enrichment indexes. Auxotrophic mutants were also isolated from strains Kyokai no. 9 and Kyokai no. 10 at enrichment indexes of more than 20,000. Of the mutants isolated, an adenine auxotroph of strain Kyokai K10 whose cells were colored red in the fermentation of sake mash was applied to produce of a new type of sake.
社団法人日本生物工学会の論文
1983-09-25