焼酎酵母の第IV染色体のMAL配列

概要

論文の詳細を見る
In the yeast Saccharomyces cerevisiae, five unlinked MAL (maltose fermentation gene) loci, MAL1,MAL2,MAL3,MAL4,and MAL6 have been identified. We surveyed the chromosomal location of the MAL homologous sequence for some industrial yeasts, and found that shochu yeasts, sake yeasts and wine yeasts isolated in Japan have a MAL homologous sequence on their chromosome IV, identified by hybridization analysis with a TRP1 gene probe. By pulse field gel electrophoresis(PFG)-Southern hybridization analysis of segregants isolated from a hybrid between a haploid spore from shochu yeast SH-4 and a mal^- laboratory strain, this homologous sequence originating in SH-4 could express maltose fermentability. Therefore, we named this sequence MAL7. These MAL^+ segregants induced maltase activity more slowly than the MAL6 segregants did. We cloned this sequence of shochu yeast SH-4 by polymerase chain reaction and found it had high homology to MAL6.
社団法人日本生物工学会の論文
1994-03-25