Oerskovia sp. CK株の生産する酵母細胞壁溶解酵素の産生条件および粗酵素の諸性質 : Oerskovia sp. ck株の生産する酵母細胞壁溶解酵素に関する研究(第2報)

概要

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The culture filtrate of the CK strain, belonging to the genus Oerskavia, exhibited high lytic activity toward logarithmic or stationary phase cells of many species of yeast, when the yeast cells were used as substrate. Culturing conditions of the CK strain giving the optium production of the cell wall lytic enzyme were investigate. It was found that glucan, the main component of yeast cell wall, and laminarin whose structure is similar to glucan, were effective induces of enzyme production. Addition of NaNO_3,KNO_3,or (NH_4)_2HPO_4 to the medium promoted the enzyme production, owing to maintenance of the broth pH around neutrality. The enzyme production was also enhanced when the medium was sterilized at pH 11.0 and readjusted to pH 7.0. This enzyme preparation showed β-1,3-glucanase, β-1,6-glucanase, mannanase, protease, and amylase activities. Optimum pH and optimum temperature of the lytic activity were 6.0-9.0 and 30°-40℃. This lytic activity was stable at pH 6.0-10.0,but was completely lost on treatment at 50℃ for 15 min. The activity was also severely inhibited by 10^<-4>M HgCl_2.
社団法人日本生物工学会の論文
1975-05-25