イソアミルアルコール耐性を指標としたエタノール耐性酵母の分離

概要

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Ethanol, which is toxic to Saccharomyces cerevisiae, inhibits the systems that transport nutrients, by dissipation of the proton motive force across the plasma membrane. Alkanols with longer chains inhibit the transport system more. We isolated highly ethanol-tolerant sake yeasts (Saccaromyces cerevisiae) selected for their isoamyl alcohol tolerance. Yeasts grown in nutrient medium containing 1% isoamyl alcohol and 5% ethanol were isolated. Some strains were viable in a 17.5% ethanol solution, in which the wild type and ethanol-tolerant yeasts isolated before could not survive. The shape of ethanol-tolerant yeasts isolated in this work stayed normal in nutrient medium containing 0.7% isoamyl alcohol, but the shape of the wild type was abnormal in the same medium. These ethanol-tolerant yeasts gained tolerance to K1 type killer yeast. The fermentative ability of ethanol-tolerant yeast was inferior to the wild type. This new method to isolate highly ethanol-tolerant yeasts was useful for the isolation of brewing yeasts.
社団法人日本生物工学会の論文
1990-03-25