Oerskovia sp. CK株による麹菌細胞壁溶解酵素の生産と粗酵素の諸性質 : Oerskovia sp. CK株の生産する麹菌細胞壁溶解酵素の利用に関する研究(第1報)

概要

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Oerskovia sp. CK was cultivated with the mycelia of koji mold(Aspergillus oryzae)and produced fungal cell wall lytic enzymes inducibly. When chitin and dry yeast were used as substrates instead of fungal mycelia, the productivity of the lytis enzyme increased greatly. When the culture conditions for enzyme production were investigated, the maximum lytic activity was found at 30℃ for 40 h in a medium containing 1% chitin powder and 0.5% dry yeast(pH 8.0). It was proved that chitinase was the main contributor to the lytic activity on fungal cell walls.Optimum temperature and pH of the lytic activity were 45℃ and 60.-7.0,respectively. The lytic activity was stable at pH 5.0-8.0,but was completely inactivated at 60℃ for 15 min.The enzyme had high lytic activity toward the cell walls of the various fungi tested except for the Zygomycetes group. This enzyme could be useful fot protoplasting of filamentous fungi.
社団法人日本生物工学会の論文
1986-11-25