清酒醪の発酵管理に関する研究 : (第3報)醸造用水の殺菌

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The effect of Diethylpyrocarbonate (DEPC) on sterilization of brewing water was studied. Results were obtained as follows : 1. The effect of DEPC on yeast kill in water was extremely influenced by pH and there was a tendency at pH 7.5-8.0 to decrease the effect.2. Concentrations of DEPC necessory to kill various microorganisms in water were 50 ppm against yeast, E. coli and B. Subtilis, 80-100 ppm against Pseud. fluorescens and 300-400 ppm against lactic acid bacteria.3. The effect of H_2O_2 on microbicidal action of DEPC was examined and it was the most effective for killing the lactic acid bacteria to add DEPC imediately after addtion of H_2O_2 to water.4. In case of sterilization of brewing water, 80-100 ppm of DEPC and 0.03-0.05% of H_2O_2 were added to it, after adjusting to about pH 6.5-5.5 with hydrochloric acid or lactic acid.
社団法人日本生物工学会の論文
1966-11-25