麹のカプロン酸エチル合成および分解活性 : 清酒に関する酵素の研究(第6報)

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There is a report about esterase in p-nitrophenyl acetate hydrolosis but no report about ethyl caproate synthesis and hydrolysis.We have studied the activity of koji in the synthesis and hydrolysis of ethyl caproate. The results obtained are as follows : 1 ) Koji had three kinds of esterase in caproate synthesis, hydrolysis, and p-nitrophenil acetate hydrolysis.2) Aspergillus oryzae RIB 647 had all extracellular and intracellular activities when cultured on YEPD liquid medium.3) The esterase in ethyl caproate synthesis was separated into two factions(I, II)by Sepharose 6B gel filtration.4) Fraction II hydrolyzed p-nitrophenyl acetate and p-nitrophenyl caproate.5) Fraction II was further separated into several fractions by DEAD-Sepharose CL-6B.6) These fractions of esterase in ethyl caproate synthesis were not always the same as those for ethyl caproate hydrolysis in the position of elution.7) An alcohol acyltransferase was found in koji.
社団法人日本生物工学会の論文
1986-07-25