Enzyme Labeling in Steroid Enzyme Immunoassays. The p-Nitrophenyl Ester Method for Alkaline Phosphatase and Glucose Oxidase Labelings
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概要
- 論文の詳細を見る
The p-nitrophenyl ester method was assesses as an enzyme labeling technique. The active ester of a carboxylated testosterone derivative was treated with alkaline phosphatase and glucose oxidase to give labeled antigens, using various molar ratios of steroid to enzyme. Satisfactory immunoreactivities with an anti-testosterone antibody in an enzyme immunoassay system were obtained with the labeled antigens prepared at pH 8.5 by the use of molar ratios higher than 30 and 10,respectively, in the alkaline phosphatase and glucose oxidase labelings.
- 公益社団法人日本薬学会の論文
- 1992-05-25
著者
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後藤 順一
Pharmaceutical Institute Tohoku University
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細田 宏
Pharmaceutical Institute Tohoku University
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福田 勝行
Pharmaceutical Institute, Tohoku University
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福田 勝行
Pharmaceutical Institute Tohoku University
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