Preparation of Haptens for Use in Immunoassays of Tetrahydro-11-deoxycortisol and Its Glucuronides

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概要

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• For the purpose of developing immunoassays of tetrahydro-11-deoxycortisol or its glucuronides, various haptenic derivatives were synthesized. The 3-hemisuccinate (15), 21-hemisuccinate (8), 3-hemiglutarate (16), 21-hemiglutarate (9) and glucuronides (19,23,25) of tetrahydro-11-deoxycortisol were prepared starting from 11-deoxycortisol 21-acetate (1). Then, antisera were elicited in two rabbits by immunization with the conjugate of tetrahydro-11-deoxycortisol 3-glucuronide (19) with bovine serum albumin. It was found that the antisera had binding affinities to enzyme-labeled antigens prepared from 15,16 and 19 ; the binding was inhibited by the glucuronide (19) in the enzyme immunoassay procedure.

• 公益社団法人日本薬学会の論文
• 1983-11-25

著者

• 南原 利夫

  Pharmaceutical Institute, Tohoku University

• 石井 和夫

  School of Health Sciences, Kyorin University

• 細田 宏

  Pharmaceutical Institute Tohoku University

• 石井 和夫

  Pharmaceutical Institute, Tohoku University

• 伊藤 裕子

  Pharmaceutical Institute Tohoku University

• 南原 利夫

  東北大学医学部薬学科

• 横浜 広光

  東北大学 薬

• 南原 利夫

  Pharmaceutical Institute Tohoku University

• 石井 和夫

  杏林大学保健学部

• 石井 和夫

  Pharmaceutical Institute Tohoku University

• 横浜 広光

  Pharmaceutical Institute, Tohoku University

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