Enzyme Labeling in Steroid Enzyme Immunoassays. Comparison of the p-Nitrophenyl Ester and N-Succinimidyl Ester Methods

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概要

• 論文の詳細を見る

• Enzyme labeling of steroids by the p-nitrophenyl ester method was investigated in comparison with the N-succinimidyl ester method. The active ester of a testosterone or 11-deoxycortisol derivative was treated with β-galactosidase and horseradish peroxidase to give labeled antigens. Various molar ratios of steroid to enzyme and pH conditions were tested. Satisfactory immunoreactivities with an anti-steroid antibody in each enzyme immunoassay system were obtained with the labeled antigens prepared at pH 8.5 by the use of molar ratios higher than 30. The enzyme labeling method should be useful in the case of polar steroids or drugs, since the p-nitrophenyl ester is relatively stable when compared with the N-succinimidyl ester.

• 公益社団法人日本薬学会の論文
• 1991-09-25

著者

• 細田 宏

  Pharmaceutical Institute Tohoku University

• 後藤 陽子

  Pharmaceutical Institute Tohoku University

• 福田 勝行

  Pharmaceutical Institute, Tohoku University

• 福田 勝行

  Pharmaceutical Institute Tohoku University

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