Preparation and Antigenic Properties of Testosterone-15α-Protein Conjugate
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概要
- 論文の詳細を見る
In order to obtain the specific antiserum used for radioimmunoassay of testosterone a new hapten-carrier conjugate was prepared from 15α-hydroxytestosterone 15-hemisuccinate by coupling with bovine serum albumin employing the mixed anhydride technique. The specificity of anti-testosterone antiserum elicited in the rabbit by immunization with this antigen was tested by cross-reaction studies with the related steroids. The results indicated that specific antiserum which is capable of differentiating testosterone from 5α-dihydrotestosterone and androstenedione to a certain extent would be produced by antigen whose steroidal moiety is coupled ot a coupled to a protein through the position remote from the inherent functional groups.
- 公益社団法人日本薬学会の論文
- 1977-11-25
著者
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南原 利夫
Pharmaceutical Institute, Tohoku University
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山下 幸和
Pharmaceutical Institute Tohoku University
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山下 幸和
日本化薬(株)医薬事業本部総合研究所
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細田 宏
Pharmaceutical Institute Tohoku University
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南原 利夫
Pharmaceutical Institute Tohoku University
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但野 恭一
Pharmaceutical Institute Tohoku University
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知野 紀子
Pharmaceutical Institute, Tohoku University
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知野 紀子
Pharmaceutical Institute Tohoku University
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