耐塩性酵母Candida famata 234Bによる L-Sorboseの還元

概要

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Halotolerant yeasts were isolated from soy sauce mash in order to study the carbohydrate metabolism. Thirty strains out of 1,000 isolates possessed activity to reduce L-sorbose to L-iditol. Twenty-one of the selected strains were identified as Candida famata. The others were identified as Debaryomyces hansenii (3 strains), Candida versatilis (3 strains), Hansenula anomala (2 strains) and Candida etchellsii (1 strain). One of these strains, C. famata 234B, which exhibited potent L-sorbose reducing activity, was used in the experiments. C. famata 234 B produced L-iditol and d-sorbitol from L-sorbose under anaerobic conditions, Whereas only L-iditol was produced from L-sorbose under anaerobic conditions. Xylitol induced the highest activity of L-iditol and D-sorbitol production. The ratio of L-iditol and D-sorbitol produced from L-sorbose was 2 : 3,and the yield of total polyol production was about 65〜70% when the concentration of L-sorbose was 5%. Under anaerobic conditions, the reduction of L-sorbose to L-iditol was accelerated by the addition of ethanol, D-glucose and D-fructose. The yield of L-iditol production from L-sorbose was about 10%, and more than 90% of the L-sorbose consumed was transformed to L-iditol. NaCl was required for growth of C. famata 234B, though the addition of NaCl to the transformation mixture inhibited polyol production.
社団法人日本生物工学会の論文
1994-07-25