食品のガス殺菌に関する研究 : (第3報) 食品殺菌剤としてのβ-プロピオラクトンの利用

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As β-propiolactone (BPL) has relatively high microbicidal activity and decomposes rapidly into the low toxic β-hydroxypropionic acid in the presence of water, increasing interest is being shown in its utilization as a sterilant for foods and other materials.The present investigation was undertaken in order to determine the microbicidal activity of BPL on several microorganisms and to confirm its effectiveness as a food sterilant.Spores of various bacteria isolated from fish sausages and their raw materials were destroyed by a BPL conccentration of 0.125-0.25% under the test conditions in Table 1.A similar concentration of BPL was required to destroy several kinds of yeast, but spores of several molds were destroyed at about one-tenth the concentration required for the destruction of bacterial spores (c.f. Fig. 1,Table2,3).Influences of temperature, pH, nutritive constituents and other factors on the sporicidal activity and hydrolytic degradation of BPL were studied.Temperature coefficients(Q_<10>) of disinfection and hydrolytic degradation were in the range between 2.0 and 3.0.The rate of hydrolytic degradation BPL became higher as pH and a concentration of sodium chloride were increased, but these factors affected the sporicidal activity of BPL only to a small degree. The sporicidal activity of BPL seemed to be enhanced in a nutrient broth more than in a phosphate buffer solution (c.f. Fig. 4). Tests of BPL for food preservation were also conducted employing fish sausage, soy cheese and other foods under actual manufacturing and storage conditions. Addition of a relatively low concentration of BPL (0.1-0.2%) was able to kill almost all contaminants existing in these foods and therefore these foods are able to be preserved for long periods at room or higher temperature if they were protected by some means (such as with air and water tight plastic casings) against secondary spoilage from the outside.
社団法人日本生物工学会の論文
1965-07-25

食品のガス殺菌に関する研究 : (第3報) 食品殺菌剤としてのβ-プロピオラクトンの利用

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