陰イオン交換樹脂・ヨード複合体の殺菌作用特性

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An anion exchange resin, polystyrene quaternary ammonium type (Amberlite IRA 401) forms a water-insoluble complex with iodine. This complex, IRC 401 showed remarkable bactericidal activity and stability compared with several anion exchange resin-iodine complexes. For example, Escherichia coli K-12 (=10^7 cells/ml) was killed entirely after passing through a 6 ml IRC 401 (2.56) column, prepared from 10 ml of IRA 401 with 2.56 g adsorbed iodine, at 20℃ and 0.02 min residence time. Surviving fractions after passing through IRC 401 column decreased as the residence time and amount of adsorbed iodine increased.One of the most interesting results was the variation in sterilizing efficiency with initial cell concentration, that is, surviving fractions after treatment by IRC 401 (2.56)column were <10^<-7> for 10^7 cells/ml, 10^<-5> for 10^8 cells/ml, and 5×10^<-1> for 10^9 cells/ml at 20℃ and 0.1 min residence time. From these results we induced an equation which could explain the characteristic sterilization by IRC 401 column.The effect of temperature on sterilization by IRC 401 was different from that by liquid iodine. In the latter, Q_<10> value was 1.42〜1.20 (10-40℃), but in the former Q_<10> was 3.22〜3.04 (10〜30℃) and at 40℃ E. coli (10^8 cells/ml) was killed entirely at 0.06 min of residence time. The activation energies for killing E.coli were 19.27 Kcal/g・mole in IRC 401 (2.56) and 5.38 Kcal/g・mole in liquid iodine.The bactericidal activity of IRC 401 (2.56) was inhibited by nutrient broth and its organic components, but this activity was restored completely by rinsing with water.It was estimated that organic substances did not react with iodine of IRC 401.
1976-03-25

陰イオン交換樹脂・ヨード複合体の殺菌作用特性

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