ガス殺菌における湿度効果発現機構

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This paper explains the reason why moisture and/or relative humidity (RH) causes variation of the sporidal activity of gaseous ethylene oxide (EO) and propylene oxide (PO) against Bacillus subtilis var. niger 4380 spores. When the desiccator containing spores was evacuated by vacuum pump for 15 sec and then filled with a gaseous sterillant and controlled RH air, the disinfectant rate constant (k) of spores with PO (830 mg/l) increased with increasing equilibration RH for humidification of spores but k with EO (200 mg/l) changed little except for 10%RH, while the amount of sterilant adsorbed by spores decrease with increasing water content of spores. With the time course of release of water from spores by evacuation, the water held by spores was divided into two parts, one on the outside of spores concerned with the adsorption of sterilant and the other on the inside of spores concerned with the reaction for death. It was estimated that evacuation within 2 min reduced only the water on the outside of spores.When spores equilibrated with 33% RH or 75% RH were exposed to EO after evacuation for 5,15 or 30 sec, the 33% RH spores were more sensitive to EO than the 75% RH spores in the case of 5 sec evacuation, but the opposite results were obtained in the case of longer evacuation. These results were in accordance with the expectation founded on the k which were calculated from the relation between the water content on the outside of spores and the adsorbed EO.The above results indicate that the difference of sensitivity of spores to gaseous sterilants at various RH is mainly due to the difference of water content of the inside of spores, and the amount of sterilant adsorbed by spores is a secondary effector. But the appearance of an optimal RH in EO sterilization is dependent on the amount of adsorbed EO because of the remarkable inhibitory effect of water on the adsorption of EO by spores.
社団法人日本生物工学会の論文
1980-03-25

ガス殺菌における湿度効果発現機構

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