細菌胞子のプロピレンオキサイド感受性に及ぼす細胞内カルシウムの影響

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Spores of Bacillus subtilis var. niger 4380 or B. subtilis 168 (try^-, try^-) prepared by cultivation in nutrient media (regular type spores; N-type spore) increased in sensitivity to propylene oxide(PO) with leakage of dipicolinic acid (DPA) and calcium (Ca) during treatment with tryptone broth (pH 1.1) or 0.01 M ethylene-diaminetetraacetic acid(EDTA) solution. The spores treated with EDTA for 24 hr at 20℃ (H-type spore), however, recovered to the almost same PO-tolerance as that of N-type spores by the treatment with 0.02 M Ca-acetate solution (pH 9.7) for 24 hr at 20℃. But little or no recovery of PO-tolerance was observed when H-type spores were treated with manganese acetate, magnesium acetate, or DPA solution. The change of PO-tolerance of spores was accompanied by variations in Ca content of spores.The difference in PO-sensitivity between N-, H-, Ca-type spore prepared from H-type spores by treatment with Ca-acetate was not attributable to differences in the water content of spores, the most important factor in gaseous PO sterilization, or to differences in PO and formaldehyde permeation into the spores.The number of single strand breaks (Bs) in H-type spore DNA was greater than in other type spore DNA when each type spore was exposed to PO under the same conditions. The regression line of log survivors on Bs was very siignificant irrespective of type of spore(α=0.005; correlation coefficient, γ=-0.99). These results indicate that Ca stabilizes DNA structure by neutralizing the electrostatic repulsion of phosphate groups in DNA. The DNA of Ca-less H-type spore will readily be broken by the alkylation of DNA with PO. This Ca effect on DNA was supported by thermodynamic, ultraviolet photochemical, and photobiological investigation.
社団法人日本生物工学会の論文
1979-07-25

細菌胞子のプロピレンオキサイド感受性に及ぼす細胞内カルシウムの影響

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