醤油酵母の細胞融合に関する研究 : 第二報プロトプラスト調製条件の再検討と遺伝子導入法の予備的検討

概要

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For improvement of fermentation activity of Zygosaccharomyces rouxii, cell fusion of the yeast with Saccharomyces cerevisiae was attempted by electric field method. The results are following: 1 For the preparation of protoplast, 2 M KCI and 2 M soribitol is the best to Z, rouxii and 1 M sorbitol is the best to S.cerevisiae as osmotic stabilizer. This observation suggests that favorable osmotic pressure is different to yeast species and mutant. 2 For the generation of protoplast, 1M KCI is the best and 2 M KCI is better to Z. rouxii as osmotic stabilizer. 3 The concentration and reaction time of Zymolyase-20 T is proved 1000 ㎍/ml and 60 min as best condition. 4 Preculture condition of yeast for protoplast preparation was surveyed and 10% NaCl addition to medium is favorable for Z. rouxii (ade-) and no addition is better for Z. rouxii (wild). 5 A trial of gene transfer of S. cerevisiae to Z. rouxii cell by electroporation method gave sixstrains having excellent fermentation activity.
三重大学の論文