Streptococcus faecalisにおけるピルビン酸酸化とそれに関連する代謝に関する研究-3-脂質欠乏培地における菌の増殖に及ぼす培養条件の影響

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Streptococcus faecalis 10 C1 was found to be unable to grow in a lipid-deficient medium when glucose was sterilized separately from the other components of the medium. On the other hand, a sufficient growth of the organism occurred not only by autoclaving glucose in the medium, but also by adding autoclaved mixtures of glucose and phosphate or alkali to the glucose-omitted medium. The growth-promoting effect of the glucose-phosphate mixture was enhanced by heating the mixture together with an appropriate amino acid, such as glycine or cystine. Lipoate, acetate or oleate was found to substitute for active substance(s) (tentatively named as "autoclave factor") which may be produced by autoclaving glucose under the conditions described above. This fact indicates that pyruvate oxidation may be essential for the growth in the medium lacking in lipid substances, their precursors, or "autoclave factor". Moreover, under a strictly anaerobic condition (in deaerated medium), the organism was able to grow without the supplementation, indicating that low levels of redox potential of the medium may be required for the growth. Catalase, ascorbic acid or FeSO4 was also found to permit the organism to grow in the absence of "autoclave factor". This fact would be consistent with this assumption. These results suggest that the effect of "autoclave factor" would be ascribed to the promotion of acetyl-CoA formation from pyruvate by providing more anaerobic conditions.
社団法人日本農芸化学会の論文

Streptococcus faecalisにおけるピルビン酸酸化とそれに関連する代謝に関する研究-3-脂質欠乏培地における菌の増殖に及ぼす培養条件の影響

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