リポ酸の検圧的定量法

概要

論文の詳細を見る
The manometric assay methods for lipoic acid by Stokstad, et al. and Gunsalus, et al. were examined and simplified as follows : Time required for preparing lipoic acid-deficient cells of Streptococcus faecalis 10 C1 was remarkably reduced. Namely, after cultivating for 8 hours in AC broth at 37℃, washed cells were directly transferred to the lipoic acid-deficient medium and highly active apo-cells were obtained within 15 hours at 37℃. For the manometric assay, reduced glutathione, adenosine and MgSO_4 were necessary for the maximum activity. Thiamine, riboflavine, glutamate and MnSO_4 could be omitted. By this simplified procedure a sufficient response of O_2 uptake was obtained to 0-10 mμg of DL-lipoic acid per vessel. The lipoic acid contents in acid digests of several natural substances were succesfully determined. Discussion was also made on an unidentified active substance(s), presumably a complex form of lipoic acid contained in yeast extract.
日本ビタミン学会の論文
1966-12-25