An Improved Chemiluminescence-Based Liposome Immunoassay Involving Apoenzyme
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概要
- 論文の詳細を見る
An improved liposome immunoassay system (LIS) combining the chemiluminescence-based LIS with an apoenzyme reactivation immunoassay system (ARIS) was developed. A low-molecular-weight co-factor, FAD (flavin adenine dinucleotide), was incorporated into liposomes instead of the high-molecular-weight enzyme GOD (glucose oxidase).FAD released from liposomes by cytolysin-hapten conjugates bound to Apo-GOD and regenerated GOD. The system allowed detection of 10 pM digoxin, the model analyte and was linear over 10 pM to 13 nM digoxin. This sensitivity was about 300 times higher than that of the homogeneous system using GOD-containing liposomes and 30 times higher than that of the heterogeneous system which we reported previously. The time required for incubation during the detection of digoxin was reduced from 20 to 3h.
- 公益社団法人日本薬学会の論文
- 1990-01-25
著者
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芳賀 信
東京理科大学薬学部
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芳賀 信
東京理大・薬
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鈴木 康男
Pharmaceutical Institute Tohoku University
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加藤 百合子
Faculty Of Pharmaceutical Sciences Science University Of Tokyo
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櫨本 紀夫
Faculty of Pharmaceutical Sciences, Science University of Tokyo
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芳賀 信
Faculty of Pharmaceutical Sciences, Science University of Tokyo
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芳賀 信
Faculty Of Pharmaceutical Sciences Sciences University Of Tokyo
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櫨本 紀夫
(Present address)Faculty of Pharmaceutical Sciences, Nagoya City University
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星野 正一郎
Faculty of Pharmaceutical Science, Science University of Tokyo
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岡田 浩江
Faculty of Pharmaceutical Science, Science University of Tokyo
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星野 正一郎
Faculty Of Pharmaceutical Science Science University Of Tokyo
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岡田 浩江
Faculty Of Pharmaceutical Science Science University Of Tokyo
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櫨本 紀夫
(present Address)faculty Of Pharmaceutical Sciences Nagoya City University
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鈴木 康男
Pharmaceutical Insitute Tohoku University
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