A Liposome Immunoassay Based on a Chemiluminescense Reaction
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概要
- 論文の詳細を見る
A new, sensitive immunoassay involving the combination of a liposome immunoassay with chemilluminescence detection is reported. Lysis of the liposomes with cytolysin-hapten conjugates released entrapped glucose oxidase. Chemiluminescence produced by reaction of the enzymatically formed hydrogen peroxide and isoluminol was monitored via an optical fiber and photomultiplier. Without a bound/free separation procedure, this chemical amplification of the immune reaction by liposome lysis and enzymatic reaction allowed detection of 1.7 ng/assay tube of digoxin, the model analyte. When liposome-coated microplates were used to separate intact liposomes from released enzyme, the sensitivity of detection was improved to 165 pg/assay tube.
- 公益社団法人日本薬学会の論文
- 1989-06-25
著者
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芳賀 信
東京理科大学薬学部
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芳賀 信
東京理大・薬
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鈴木 康男
Pharmaceutical Institute Tohoku University
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加藤 百合子
Faculty Of Pharmaceutical Sciences Science University Of Tokyo
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櫨本 紀夫
Faculty of Pharmaceutical Sciences, Science University of Tokyo
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芳賀 信
Faculty of Pharmaceutical Sciences, Science University of Tokyo
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芳賀 信
Faculty Of Pharmaceutical Sciences Sciences University Of Tokyo
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櫨本 紀夫
(Present address)Faculty of Pharmaceutical Sciences, Nagoya City University
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星野 正一郎
Faculty of Pharmaceutical Science, Science University of Tokyo
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中村 徹
Faculty of Pharmaceutical Sciences, Science University of Yokyo
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星野 正一郎
Faculty Of Pharmaceutical Science Science University Of Tokyo
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櫨本 紀夫
(present Address)faculty Of Pharmaceutical Sciences Nagoya City University
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鈴木 康男
Pharmaceutical Insitute Tohoku University
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中村 徹
Faculty Of Pharmaceutical Sciences Science University Of Yokyo
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