Characteristics of Anion Transport Channels in the Human Erythrocyte. I. Interactions between Eosin 5-Isothiocyanate and Band 3 Proteins
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概要
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The interaction between eosin 5-isothiocyanate (EITC) and human erythrocyte ghosts has been studied by means of fluorescence and absorption difference spectroscopy. The fluorescence quenching data of the ghost-EITC system showed a biphasic change. EITC molecules bound to band 3 proteins and adsorbed on ghost membranes are responsible for this biphasic change. Binding of EITC molecules to the ghosts leads to a redshift and hypochromism in the visible region. Consequently, the difference spectrum shows a positive peak and two negative ones at 540,517,and 483 nm from the longer wavelength side. The intensities of these peaks change heterogeneously under various conditions, reflecting the contributions of xanthene and phenyl chromophores bound to band 3. It was considered that the conformational structure of EITC binding sites is sensitive to temperature, showing a transition at about 30℃. The reactivity of EITC molecules with band 3 at temperatures below 30℃ is different from that above 30℃. It is inferred that the EITC binding sites on band 3 are identical with the modifier sites.
- 社団法人日本薬学会の論文
- 1985-09-25
著者
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佐藤 幸夫
Faculty Of Pharmaceutical Sciences Tohoku University
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鈴木 康男
Pharmaceutical Institute, Tohoku University
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佐藤 幸夫
Pharmaceutical Institute, Tohoku University
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千葉 忠彦
Pharmaceutical Institute, Tohoku University
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千葉 忠彦
Pharmaceutical Institute Tohoku University
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鈴木 康男
Pharmaceutical Institute Tohoku University
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佐藤 幸夫
東北大学薬学部
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Sato Yukio
Faculty Of Pharmaceutical Science Tohoku University
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鈴木 康男
Pharmaceutical Insitute Tohoku University
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