Spectrofluorometric Determination of FUT-175 (Nafamstat Mesilate) in Blood Based on Trypsin-Inhibitory Activity
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概要
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FUT-175 (nafamstat mesilate) is a novel synthetic protease-inhibiting agent which has potent, reversible and selective inhibitory activity against trypsin-like serine proteases. A determination method for FUT-175 in biological materials, particularly in blood, was established based on the potent trypsin-inhibitory activity of this drug. The procedure consists of two steps : treatment of blood specimens with formic acid-containing ethanol for deproteinization and extraction of FUT-175 with minimal hydrolysis, and a clean-up with n-pentane for removal of remaining blood constituents such as phospholipids, fats and any fluorescent materials. This method shows good reproducibility and sensitivity ; the detection limit was as low as 1 ng of FUT-175/ml blood.
- 公益社団法人日本薬学会の論文
- 1985-05-25
著者
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青山 卓夫
Research Laboratories Torii & Co. Ltd.
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青山 卓夫
鳥居薬品株式会社研究所
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鈴木 康男
Pharmaceutical Institute Tohoku University
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佐々木 洋明
Research Laboratories Torii & Co. Ltd.
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渋谷 正興
Research Laboratories, Torii & Co., Ltd.
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鈴木 康男
Pharmaceutical Insitute Tohoku University
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渋谷 正興
Research Laboratories Torii & Co. Ltd.
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