舌乳頭部位に存在するエステラーゼ類の研究(第2報) : コリンエステラーゼ類の電気泳動による分離

概要

論文の詳細を見る
There are histochemical evidences that some of esterases including cholinesterases are localized at the basement of taste buds of vallate papilla of rat tongue and foliate papilla of rabbit tongue. The present article reports the separation of esterases in taste papilla by polyacrylamide-gel thin-layer electrophoresis using α-naphthyl acetate as substrate and fast blue BB salt as coupler. For extraction of tissue esterases, presence of 0.5% Triton-X-100 seems to be desirable and its presence had no effect on the electrophoretic mobility. This fact suggests that some enzymes are firmly bound to the membrane and solubilized by surface active agents. It has been possible to separate ten active bands from rat vallate papilla and eleven active bands from rabbit foliate papilla by the technique described. Of these esterases, two active bands which have lower electrophoretic mobility and detected only after Triton-X-100 treatment were found to exhibit cholinesterase activity.
社団法人日本薬学会の論文
1969-04-25