Somatic Hybrids between Lycopersicon esculentum and Lycopersicon chmielewskii

スポンサーリンク

概要

• 論文の詳細を見る

• Interspecific somatic hybrids were obtained by electric protoplast fusion of cotyledon protoplasts of Lycopersicon esculentum Mill. cv. Kyoryokutoko treated with iodoacetamide (IOA) and suspension-culture-derived protoplasts of L. chmielewskii (LA1330). A multiple-step selection procedure was used in selecting the hybrids depending on IOA treatments and a culture medium that only allows cotyledon protoplasts to regenerate. Plants were regenerated on the calli derived from fused protoplasts. After acclimation, the hybrid plants with fruits through self-pollination were obtained. Identification of the regenerated putative hybrid plants was carried out by morphological, chromosomal and random amplified polymorphic DNA (RAPD) analysis, as well as restriction fragment length polymorphism (RFLP) comparison of parental and hybrid plants. The hybrids showed unique morphologies in leaf, flower and stem compared with that of two fusion partners, 2n = 4x = 48, 47, 69-72 chromosomes, both of the parental-specific RAPD bands, and genomic recombination and elimination occurred in mitochondria level. Cell fusion technique shows its new potential in foreign gene transformation between L. esculentum and L. chmielewskii.

• 日本植物細胞分子生物学会の論文
• 2002-12-03

著者

• INOUE Makoto

  Faculty of Pharmaceutical Sciences, Nagoya City University

• Sato Yukio

  Toyama Prefectural Univ. Toyama Jpn

• Sato Yukio

  Faculty Of Pharmaceutical Science Tohoku University

• Sato Yukio

  Faculty Of Agriculture Miyazaki University

• Adachi Taiji

  Faculty Of Agriculture University Of Miyazaki

• Sato Y

  Toyama Prefectural University

• Chen Lanzhuang

  Gene Research Center Miyazaki University

• MURAI Kaoru

  Faculty of Agriculture, Miyazaki University

• KANEKO Yuko

  Faculty of Agriculture, Miyazaki University

• Murai Kaoru

  Faculty Of Agriculture Miyazaki University

• Kaneko Yuko

  Faculty Of Agriculture Miyazaki University

• Inoue Makoto

  Faculty Of Agriculture Miyazaki University

• Adachi Taiji

  Faculty Of Agriculture Miyazaki University

関連論文

• Hemoglobin Denaturation Caused by Surfactants
• Interaction of Eosin-5-maleimide with Band 3 of Human Erythrocytes
• Mechanism of Hypotonic Hemolysis of Human Erythrocytes
• Participation of Band 3 Protein in Hypotonic Hemolysis of Human Erythrocytes
• Color Chenge of New Coccine in a Lysozyme-Alimezine-Trimetoquinol Syrup : Optical Spectroscopic and Kinetic Analyses
• Electron Spin Resonance Studies of Phosphatidylcholine Interacted with Cholesterol and with a Hopanoid in Liposomal Membrane
• Interaction of Pentoxifylline with Human Erythrocytes. III. Comparison of Fluidity Change of Erythrocyte Membrane Caused by S-Adenosyl-L-methionine with That by Pentoxifylline
• Interaction of Liposomes with Human Erythrocytes
• Interaction of Pentoxifylline with Human Erythrocytes. II. : Effects of Pentoxifylline on the Erythrocyte Membrane
• Interaction of Pentoxifylline with Human Erythrocytes. I. : Interaction of Xanthine Derivatives with Human Erythrocyte Ghosts
• Characterization of Secondary Structure of Neocarzinostain Apoprotein
• Alteration of Immunolysis Reaction on Liposome Membrane by Various Cholesterol Analogues
• CHARACTERIZATION OF IMMUNILIPOSOME MEMBRANE : ESP SPECTROSCOPIC ANALYSIS OF THE EFFECT OF CHOLESTEROL DERIVATIVES IN MEMBRANE
• Highly Sensitive Immunoliposome Assay of Theophylline
• Optical Resolution of Drugs by Cyclodextrin Complexation
• Characteristics of Anion Transport Channels in the Human Erythrocyte. I. Interactions between Eosin 5-Isothiocyanate and Band 3 Proteins
• Biphasic Photolysis of Riboflavin. III. Effects of Ionic Strength on the Photolysis
• Development of Basic Technologies for Drop-Tower Experiments on Vertebrates
• A new species of Brasiliomyces (Erysiphaceae) on Dalbergia cultrata var. cultrata from Thailand
• THE STABILIZING ACTION OF HYDROXYPYRUVALDEHYDE PHENYLOSAZONE AND ITS DERIVATIVES ON BOVINE ERYTHROCYTE MEMBRANE
• Tiny keratotic brown lesions on the interdigital web between the toes of a healthy man caused by Curvularia species infection and a review of cutaneous Curvularia infections
• LONG-TERM ADMINISTRATION OF "SHO-SAIKO-TO" INCREASES CYTOCHROME P-450 mRNA LEVEL IN MOUSE LIVER
• Biphasic Photolysis of Riboflavine with a Low-intensity Light Source
• The Mechanism of Polyembryonic Seed Set in Paspalum notatum
• The Mechanisms of Appearance of Aposporous Initial Cell and Apomictic Embryo Sac Formation in Paspalum notatum
• Embryo Sac Morpho-structural Analysis of Developmental Pathways of Fertilization Failure, Seed Abortion and Poor Germination in Cassava(Manihot esculenta Crantz)^*
• Molecular phylogenetic analyses reveal a close relationship between powdery mildew fungi on some tropical trees and Erysiphe alphitoides, an oak powdery mildew
• A new subgenus, Microidium, of Oidium (Erysiphaceae) on Phyllanthus spp.
• Erysiphe wadae: a new species of Erysiphe sect. Uncinula on Japanese beech
• Phylogenetic relationships of Microsphaera and Erysiphe section Erysiphe (powdery mildews) inferred from the rDNA ITS sequences
• Phylogenetic analysis and predicted secondary structures of the rDNA internal transcribed spacers of the powdery mildew fungi (Erysiphaceae)
• On-Line Signature Verification by Adaptively Weighted DP Matching (Special Issue on Character Recognition and Document Understanding)
• 原子間力顕微鏡でみる細胞構造
• Biphasic Photolysis of Riboflavin. II. Effect of Gelatin on the Photolysis
• Somatic Hybrids between Lycopersicon esculentum and Lycopersicon chmielewskii
• Antioxidant and Immuno-Enhancing Effects of Echinacea purpurea(Pharmacology)
• Isolation of Bovine Serum Albumin Fragment P-9 and P-9-Mediated Fusion of Small Unilamellar Vesicles
• Ultrastructural Studies of Embryo Abortion in Buckwheat (Fagopyrum esculentum) as a Heat-stress
• Preliminary Isolation of Viable Protoplasts from Egg Cells in Facultative Apomictic Guineagrass (Panicum maximum)
• Effect of Harvest Seasons on the Efficiency of Ovary Culture in Panicum maximum
• A Simple and Efficient Culture of Leaflets for Plant Regeneration in Guineagrass (Panicum maximum)
• Establishment of Embryogenic Suspension Culture Derived from Leaflets of Sexual Bahiagrass (Paspalum notatum) with Regeneration Ability in Long Term
• Somatic Embryogenesis and Plant Regeneration from Leaflets of "Nanou"Bahiagrass
• RVB States for Two-Dimensional Antiferromagnets
• Specific Heat of the Two-Dimensional ± J Ising Model

もっと見る 閉じる

スポンサーリンク