A Simple and Efficient Culture of Leaflets for Plant Regeneration in Guineagrass (Panicum maximum)

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概要

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• A simple and efficient culture for plant regeneration was established from the leaflet tissue of Panicum maximum. The vegetative leaflets were excised from terminal tillers arising at the end of stolons and cultured for callus formation on Murashige and Skoog (MS) medium supplemented with 2, 4-dichlorophenoxyacetic acid. After 30 days of culture, compact callus formed. And after 60 days, white embryogenic calli were induced with wave-like-shapes. When the embryogenic calli were transferred onto MS medium supplemented with kinetin and giberellic acid for regeneration, they developed and formed multiple shoots. The rooted plants were obtained within 4 months. All of the regenerants were successfully grown to maturity.

• 日本植物細胞分子生物学会の論文
• 2002-03-01

著者

• Adachi T

  Faculty Of Agriculture University Of Miyazaki

• Adachi Taiji

  Faculty Of Agriculture University Of Miyazaki

• Guan Liming

  Faculty Of Agriculture University Of Miyazaki

• Chen L

  Faculty Of Horticulture Minami Kyushu University

• Chen Lanzhuang

  Gene Research Center Miyazaki University

• GUAN Liming

  Gene Research Center, Miyazaki University

• OKABE Reiji

  Faculty of Agriculture, Miyazaki University

• ADACHI Taiji

  Osaka Prefecture University

• Okabe Reiji

  Faculty Of Agriculture Miyazaki University

• Adachi T

  Miyazaki Univ. Miyazaki Jpn

関連論文

• The Mechanism of Polyembryonic Seed Set in Paspalum notatum
• The Mechanisms of Appearance of Aposporous Initial Cell and Apomictic Embryo Sac Formation in Paspalum notatum
• Embryo Sac Morpho-structural Analysis of Developmental Pathways of Fertilization Failure, Seed Abortion and Poor Germination in Cassava(Manihot esculenta Crantz)^*
• Somatic Hybrids between Lycopersicon esculentum and Lycopersicon chmielewskii
• Ultrastructural Studies of Embryo Abortion in Buckwheat (Fagopyrum esculentum) as a Heat-stress
• Preliminary Isolation of Viable Protoplasts from Egg Cells in Facultative Apomictic Guineagrass (Panicum maximum)
• Effect of Harvest Seasons on the Efficiency of Ovary Culture in Panicum maximum
• A Simple and Efficient Culture of Leaflets for Plant Regeneration in Guineagrass (Panicum maximum)
• Establishment of Embryogenic Suspension Culture Derived from Leaflets of Sexual Bahiagrass (Paspalum notatum) with Regeneration Ability in Long Term
• Somatic Embryogenesis and Plant Regeneration from Leaflets of "Nanou"Bahiagrass
• Cytological Studies of Hybrids between Aneuploid and Eu-diploid Japanese Garden Iris (Iris ensata Thunb.)

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