Identification of the Decomposition Products of Hydralazine Hydrazones with Three Endogenous Ketones and Kinetic Study of the Formation of 3-Methyl-s-triazolo [3,4-a] phthalazine from Hydralazine and Pyruvic Acid

概要

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The decomposition products of hydrazones of hydralazine with pyruvic acid, acetone and α-ketoglutaric acid were studied. The pyruvic acid hydrazone and acetone or α-ketoglutaric acid hydrazone were converted to 3-methyl-s-triazolo [3,4-a] phthalazine (MTP) and phthalazinone, respectively, both of which are known hydralazine metabolites. New high pressure liquid chromatographic (HPLC) methods for determining hydralazine and MTP were developed. The formation of MTP and the disappearance of hydralazine at a physiological concentration ratio and pH at 37° were studied by the HPLC methods. The formation of MTP followed second-order kinetics, and its rate constant was determined.
公益社団法人日本薬学会の論文
1980-11-25