Microbial Synthesis of 5-Fluorouridine Monophosphate and 5-Fluoro-2'-Deoxyuridine Monophosphate

概要

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A microbial nucleoside phosphotransferase reaction was applied for the conversion of 5-fluorouridine (FUR) and 5-fluoro-2'-deoxyuridine (FUdR) into the corresponding fluorinated nucletide analogs on a preparative scale. Under optimized conditions using washed cells of Serratia marcescens IFO 3046 and p-nitrophenyl phosphate as a phosphate donor, FUR and FUdR were converted to the phosphorylated form with conversion yields of 81.5% and 69.0%, respectively. Chemical and enzymatic analyses suported identification of the two fluorinated nucleotide products as 5-fluorouridine 5'-monophosphate (FUMP) and 5-fluoro-2'-deoxyuridine 5'-monophosphate (FdUMP). The phosphotransferase reaction product, FUMP, could be directly used for the successive synthesis of 5-fluorouridine diphosphate galactose using air-dried cells of Candida satioana IFO 0768.
公益社団法人日本生物工学会の論文
1994-06-25