Serotonin Induces Apoptosis in PGT-β Pineal Gland Tumor Cells

元データ 2000-09-01 社団法人 日本薬理学会

概要

Serotonin−induced neuronal cell death has been implicated as a possible cause of neurodegenerative and neuropsychiatric disorders.To investigate the involvement of serotonin−induced apoptosis as a potential mechanism in the pathophysiology of serotonin−related diseases, MTT(3−(4, 5−dimethylthiazol−2−yl)−2, 5−diphenyltetrazolium bromide)assay, DAPI(4’, 6−diamidino−2−phenylindole)staining, TUNEL(terminal deoxynucleotidyl transferase−mediated dUTP−biotin nick end labeling)assay and flow cytometric analysis were performed using the immortalized pineal cell line PGT−β.Through morphological and biochemical analyses, it was demonstrated that cell death induced by serotonin in PGT−β cells shows classic apoptotic features.These data suggest that serotonin induces apoptosis in PGT−β cells.

著者

Kim C‐j Department Of Physiology College Of Medicine Kyung Hee University
Kim C‐j Kyung Hee Univ. Seoul Kor
KIM Chang-Ju Department of Physiology, College of Medicine, Kyung Hee University
CHUNG Joo-Ho Departments of Pharmacology, College of Medicine, Kyung Hee University
YIM Sung-Vin Department of Pharmacology, College of Medicine, Kangwon National University
KIM Kyung-Hwan Inje University, Sanggye-Baek Hospital
Chung J‐h Departments Of Pharmacology College Of Medicine Kyung Hee University
Yim Sung-vin 韓国
Chung Joo-ho Departments Of Pharmacology College Of Medicine Kyung Hee University
Chung Joo-ho Department Of Pharmacology College Of Medicine Kohwang Medical Research Institute Kyung Hee Universi
Kim Chang-ju Department Of Physiology College Of Medicine Kyung Hee University
Kim Kyung-hwan Inje University Sanggye-baek Hospital
Kim Chang-ju Dep. Of Physiology Kyung Hee Univ.
Yim Sung-vin Department Of Pharmacology College Of Medicine Kohwang Medical Research Institute Kyung Hee Universi

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