Nitric Oxide Induces Apoptosis in Mouse C2C12 Myoblast Cells
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概要
- 論文の詳細を見る
To investigate whether nitric oxide (NO) induces apoptosis in myoblast cells, the effect of the sodium nitroprusside (SNP), NO donor, on the apoptosis of mouse C2C12 myoblast cells was examined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, flow cytometry, 4,6-diamidino-2-phenylindole (DAPI) staining, terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL) assay, DNA fragmentation assay, reverse transcription-polymerase chain reaction (RT-PCR), Western blot analysis, and caspase-3 enzyme assay. Mouse C2C12 myoblast cells treated with SNP exhibited several apoptotic features. SNP increased p53 expression and bax expression. SNP also enhanced caspase-3 enzyme activity. The data show that NO may induce apoptotic cell death in myoblast cells through the activation of p53-, bax-, and caspase-dependent intracellular death-related pathways.
- 社団法人 日本薬理学会の論文
- 2005-03-20
著者
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Kim C‐j
Department Of Physiology College Of Medicine Kyung Hee University
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Kim C‐j
Kyung Hee Univ. Seoul Kor
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KIM Eun-Kyung
College of Medicine, Kohwang Medical Research Institute, Kyung Hee University
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JANG Mi-Hyeon
Department of Physiology, College of Medicine, Kyung Hee University
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LEE Myoung-Hwa
Department of Physiology, College of Medicine, Kyung Hee University
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KIM Chang-Ju
Department of Physiology, College of Medicine, Kyung Hee University
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Kim Chang-ju
韓国
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Kim Ee-hwa
Department Of Physiology College Of Medicine Kyung Hee University
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Kim Ee-hwa
韓国
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Lee M‐h
Department Of Physiology College Of Medicine Kyung Hee University
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KIM Eun-Kyung
Department of Physiology, College of Medicine, Kyung Hee University
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HAN Sang-Wan
Department of Physical Therapy, Kwangju Health College
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CHO Seong-Yeon
Department of Physiology, College of Medicine, Kyung Hee University
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Lee Myoung-hwa
Department Of Physiology College Of Medicine Kyung Hee University
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Jang M‐h
Department Of Physiology College Of Medicine Kyung Hee University
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Cho Seong-yeon
Department Of Physiology College Of Medicine Kyung Hee University
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Han Sang-wan
Department Of Physical Therapy Kwangju Health College
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Jang Mi-hyeon
Department Of Physiology College Of Medicine Kyung Hee University
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Kim Chang-ju
Department Of Physiology College Of Medicine Kyung Hee University
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Kim Chang-ju
Dep. Of Physiology Kyung Hee Univ.
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Kim Eun-kyung
Department Of Diagnostic Radiology Chosun University Medical School
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