Bee Venom Induces Apoptosis and Inhibits Expression of Cyclooxygenase-2 mRNA in Human Lung Cancer Cell Line NCI-H1299

元データ 2003-02-01 社団法人 日本薬理学会

概要

To investigate whether bee venom (BV) induces apoptosis, the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, terminal deoxynucleotidyl transferase- mediated dUTP nick end-labeling assay, 4,6-diamidino-2-phenylindole staining, flow cytometric analysis, and DNA fragmentation assay were performed on NCI-H1299 lung cancer cells treated with BV. Through morphological and biochemical analyses, it was demonstrated that NCI-H1299 cells treated with BV exhibit several features of apoptosis. In addition, reverse transcription-polymerase chain reaction and prostaglandin E2 (PGE2) immunoassay were performed to verify whether BV possesses an inhibitory effect on the expression of cyclooxygenase (COX) and PGE2 synthesis. Expression of COX-2 mRNA and synthesis of PGE2 were inhibited by BV. These results suggest the possibility that BV may exert an anti-tumor effect on human lung cancer.

著者

Kim C‐j Department Of Physiology College Of Medicine Kyung Hee University
Kim C‐j Kyung Hee Univ. Seoul Kor
KIM Eun-Kyung College of Medicine, Kohwang Medical Research Institute, Kyung Hee University
JANG Mi-Hyeon Department of Physiology, College of Medicine, Kyung Hee University
SHIN Min-Chul Department of Physiology, College of Medicine, Kyung Hee University
KIM Chang-Ju Department of Physiology, College of Medicine, Kyung Hee University
KIM Ee-Hwa Department of Acupoint & Meridian, College of Oriental Medicine, Semyung University
LIM Sabina Research Group of Pain and Neuroscience in Vision 2000 Project, East-West Medical Research Institute
HAN Seung-Moo Research Group of Pain and Neuroscience in Vision 2000 Project, East-West Medical Research Institute
PARK Hi-Joon Research Group of Pain and Neuroscience in Vision 2000 Project, East-West Medical Research Institute
SHIN Insop Research Group of Pain and Neuroscience in Vision 2000 Project, East-West Medical Research Institute
LEE Ji-Suk Research Group of Pain and Neuroscience in Vision 2000 Project, East-West Medical Research Institute
KIM Kyoung-Ah Research Group of Pain and Neuroscience in Vision 2000 Project, East-West Medical Research Institute
Lee Jong-soo Department Of Physiology College Of Medicine Kyung Hee University
Kim Ee-hwa Department Of Physiology College Of Medicine Kyung Hee University
Kim Ee-hwa 韓国
Lee J‐s Department Of Physiology College Of Medicine Kyung Hee University
Shin H‐d College Of Nursing Science Kyung Hee University
Park H‐j Korea Univ. Seoul Kor
Lee Jong-sung Biotoxtech Co. Ltd.
Lee Jong-sung 韓国
Jang M‐h Department Of Physiology College Of Medicine Kyung Hee University
Shin M‐c Department Of Physiology College Of Medicine Kyung Hee University
Shin Min-chul Department Of Physiology College Of Medicine Kyung Hee University
Kim Kyung-ah Research Group Of Pain And Neuroscience In Vision 2000 Project East-west Medical Research Institute
Han Seung-moo Research Group Of Pain And Neuroscience In Vision 2000 Project East-west Medical Research Institute
Shin Insop Research Group Of Pain And Neuroscience In Vision 2000 Project East-west Medical Research Institute
Jang Mi-hyeon Department Of Physiology College Of Medicine Kyung Hee University
Kim Chang-ju Department Of Physiology College Of Medicine Kyung Hee University
Lim Sabina Research Group Of Pain And Neuroscience In Vision 2000 Project East-west Medical Research Institute
Lee J Kyung Hee Univ. Seoul Kor
Kim Chang-ju Dep. Of Physiology Kyung Hee Univ.
Park Hi-joon Research Group Of Pain And Neuroscience In Vision 2000 Project East-west Medical Research Institute
Lee Ji-suk Research Group Of Pain And Neuroscience In Vision 2000 Project East-west Medical Research Institute
Lee J Research Group Of Pain And Neuroscience In Vision 2000 Project East-west Medical Research Institute

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