Molecular cytogenetical and phylogenetical studies of <I>Lachenalia congesta</I> (Asparagaceae)
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概要
- 論文の詳細を見る
The ploidy level and the phylogenic position of <I>Lachenalia congesta</I> W. F. Barker (Asparagaceae) were analyzed by karyomorphological, molecular cytogenetical and molecular phylogenetical studies. The chromosome number of <I>L. congesta</I> was 2n=28, and these chromosomes showed bi-modal karyotype with four large sized chromosomes and 24 small sized chromosomes. The ploidy level of this species was diploid, because each two chromosomes made 14 pairs on the karyotype analysis by acetoorcein staining, DAPI staining and FISH methods. In addition two signals of 18S rDNA sites were detected by the FISH method supported this assumption. All 28 chromosomes of this species did not show any clear DAPI-positive bands and four signals of 5S rDNA sites were detected near the centromeres of small sized chromosomes. These characters were quite different from the characters observed by karyomorphological and molecular cytogenetical studies on common <I>Lachenalia</I> species with basic chromosome number of x=7 or 8. The data from molecular phylogenetic analysis of internal transcribed spacer (ITS) region supported this hypothesis.
著者
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Hamatani Shuichi
The Hiroshima Botanical Garden
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Kondo Katsuhiko
Laboratory Of Plant Chromosome And Gene Stock Faculty Of Science Hiroshima University
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Masuda Yu
Laboratory Of Plant Chromosome And Gene Stock Graduate School Of Science Hiroshima University
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Tagashira Norikazu
Department of Liberal Arts, Faculty of Liberal Arts, Hiroshima Jogakuin University, 4-13-1 Ushita Higashi, Higashi-Ku, Hiroshima-City, Hiroshima 732-0063, Japan
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Tagashira Norikazu
Department of Life Design and Information Science, Faculty of Human Life Science, Hiroshima Jogakuin University,
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Uchida Mariko
Department of Life Design and Information Science, Faculty of Human Life Science, Hiroshima Jogakuin University,
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Kusaba Makoto
Laboratory of Plant Chromosome and Gene Stock, Graduate School of Science, Hiroshima University
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