Isolation of a particular chromosome of Ajania remotipinna in a chromosome complement of an artificial F_1 hybrid of Dendranthema lavandulifolia × Ajania remotipinna by use of genomic in situ hybridization

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The maternal Dendranthema lavandulifolia (2n=18) and the paternal Ajania remotipinna (2n=18) were artificialy, successfully crossed. The hybrid seeds were germinated and developed up to plants (2n=18) in axenic culture in vitro. Karyotyping in combination with fluorescence in situ hybridization by the pTa71 probe showed eight rDNA signals in the chromosome complement of D. lavandulifolia, four in that of A. remotipinna, and the intermediate number of six in that of their artificial hybrid plants. The signal by the Arabidopsis-type telomere sequence repeats were located commonly at the terminal region of the chromosomes in the three taxa. Fluorescence genomic in situ hybridization with a blocking DNA detected a major difference between the genome of D. lavanduhfolia and that of A. remoapinna in the genome of their artificial F_1 hybrid ; 17 yellow-colored chromosomes fluoresced by Avidin-FITC, that were within the property of the common total genome as all could be hybridized with the probe of labelled total genomic DNA of D. lavandulifolia, and the other one chromosome counterstained by propidium iodide, that was not hybridized with the probe. The latter chromosome could be originated from A. remotipinna.
財団法人染色体学会の論文