Evidences of intergeneric somatic-hybrids between Dendranthema grandiflora cv. 'Shuho-no-chikara' and Artemisia sieversiana and their chromosomal mutations by using fluorescent in situ hybridization and genomic in situ hybridization
スポンサーリンク
概要
- 論文の詳細を見る
Fluorescent in situ hybridization (FISH) and genomic in situ hybridization (GISH) were applied to the somatic chromosomes of the intergeneric hybrids between Dendranthema grandiflora (Ramat.) Kitamura cv. 'Shuho-no-chikara' and Artemisia sieversiana J.F. Ehrh. ex. Willd) produced by electrofusion of mesophyll protoplasts. GISH confirmed the somatic hybridization and separated the chromosomes of the two parents in two colors in the chromosome complement of the somatic hybrid after hybridization with the labeled total genomic DNA probe of D. grandiflora. Two translocations were detected in the chromosomes of the hybrid. Simultaneous bicolor of FISH and GISH showed that 13 signals of the 45S rDNA sites were located on the chromosomes of D. grandiflora, while two signals were located on two chromosomes of A. sieversiana in the chromosome complement of the hybrid.
- 財団法人染色体学会の論文
著者
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Kondo Katsuhiko
Laboratory of Plant Genetics and Breeding Science, Department of Agriculture, Faculty of Agriculture
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Abd El-Twab
Department of Botany and Microbiology, Faculty of Science
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Kondo Katsuhiko
Laboratory Of Plant Chromosome And Gene Stock Faculty Of Science Hiroshima University
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Magdy Hussein
Department Of Botany Faculty Of Science El-minia University
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Shinoyama Harue
Fukui Agricultural Experiment Station
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